水蔓菁总黄酮对大鼠前列腺增生模型的影响

Effects of Total Flavonoids from Bastard Speedwell on Benign Prostatic Hyperplasia Rats Models

关键词:  水蔓菁  总黄酮  大鼠前列腺增生模型  睾酮  雌二醇  表皮生长因子

Key words:  Bastard Speedwell  total flavonoids  rat model of benign prostatic hyperplasia  testosterone  estradiol  epidermal growth factor

摘要:

目的 探讨水蔓菁总黄酮对去势加丙酸睾酮致前列腺增生大鼠模型的作用特点。方法 大鼠摘除双侧睾丸后第8天开始，连续30 d皮下注射丙酸睾酮4 mg·kg^-1·d^-1，造大鼠前列腺增生模型，将模型大鼠分为5组，分别灌服120，60，30 mg·kg^-1的水蔓菁总黄酮液、300 mg·kg^-1癃闭舒胶囊混悬液和同体积生理盐水，另设一组空白对照组给同体积生理盐水；每天给药1次，连续给药30 d。末次给药1 h后处死大鼠，取前列腺称重后，放射免疫法测定前列腺组织睾酮、雌二醇水平，酶联免疫法测定表皮生长因子水平；前列腺组织切片，观察组织形态。结果 水蔓菁总黄酮可显著降低前列腺增生模型大鼠前列腺湿重及前列腺指数，显著降低前列腺中睾酮和表皮生长因子水平，显著升高雌二醇水平，使前列腺腺体明显缩小、间质增多、腺体体密度下降、比表面值增大。结论 水蔓菁总黄酮具有抗前列腺增生作用，可能与降低雄激素水平，改善紊乱的雌、雄激素比例，降低前列腺中刺激性生长因子水平等有关。

Abstract:

OBJECTIVE To explore action mechanism of total flavonoids from Bastard Speedwell (TFBS) on prostatic hyperplasia rats model induced by castration and testosterone propionate. METHODS Removing of bilateral testes in rats, and subcutaneous injecting of testosterone propionate 4 mg·kg^-1·d^-1 consecutive for 30 d from the 8th day of castration, established rat model of benign prostatic hyperplasia. The model rats were divided into 5 groups, respectively were fed with 120, 60, 30 mg·kg^-1 of TFBS water solution, Longbishu capsule suspension and the same volume of normal saline solution. Separate a group of blank control group was fed with the same volume of normal saline solution. Every group was given daily administration and consecutive for 30 days. One hour after the last administration, rats were killed, taken after weighing the prostate, measured the levels of testosterone and estradiol in the prostate tissue homogenate by RIA, measured the level of epidermal growth factor in the prostate tissue homogenate by enzyme-linked immunosorbent assay, other part of prostate tissue in formalin solution fixed, sliced, examination of prostate tissue in each group cytological morphology. RESULTS TFBS could significantly reduce the prostate wet weight and prostate index in rat model with benign prostatic hyperplasia, significantly reduce the levels of testosterone and epidermal growth factor, and significantly increase estradiol levels in the prostate of rat model. Significantly reduce the prostate gland, increase mesenchyma, decrease density of glands, and increase surface area value. CONCLUSION TFBS has good actions of anti-benign prostatic hyperplasia, the action mechanism may be related with reducing androgen levels, improving the disordered ratio of estrogen and androgen, and reducing the level of irritation growth factor in prostate and so on.