荧光分析法测定兔血浆中蛇葡萄素浓度

Development of a Fluorescence Analysis Method for Determining Ampelopsin in Rabbit Plasma

关键词:  蛇葡萄素  荧光分析法  生物分析  药代动力学

Key words:  ampelopsin  fluorescence analysis  bioanalysis  pharmacokinetics

摘要:

目的 建立一种用于测定兔血浆中蛇葡萄素浓度的荧光分析方法。方法 经单剂量静脉给药后，收集家兔血样并经乙酸乙酯萃取，加入三氯化铝溶液后在激发波长442 nm和发射波长502 nm下进行血药浓度检测。结果 血浆中蛇葡萄素浓度在0.5~36.6 μg·mL^-1内与荧光强度呈良好线性关系，r=0.999 6。检测限为70 ng·mL^-1，日内、日间精密度在98.78%~103.57%之间，RSD均小于8.25%。结论 该方法简便、快速、准确，可用于蛇葡萄素的药代动力学研究。

Abstract:

OBJECTIVE To establish a fluorescence analysis method for determining ampelopsin in rabbit plasma. METHODS After intravenous administration with ampelopsin at a single dose, the blood samples were collected and extracted with ethyl acetate. Ampelopsin concentrations were determined with fluorescence detection exciting at 442 nm and emission at 502 nm. RESULTS The calibration curve showed a linear response over the concentration range of 0.5-36.6 μg·mL^-1(r=0.999 6). The limit of detection was 70 ng·mL^-1. The recovery was between 98.78% and 103.57%. The relative standard deviations of intra-day and inter-day were less than 8.25%. CONCLUSION A rapid, simple and accurate method has been successfully applied to a pharmacokinetics study of ampelopsin in rabbits.