鱼皮胶原蛋白水解液中小分子寡肽的分子量分布测定

Determination of the Molecular Weight Distribution of Small Molecule Oligopeptide in Fish Skin Collagen Hydrolyzate

关键词:  寡肽  分子量分布  测定方法

Key words:  oligopeptide  molecular weight distribution  determination

摘要:

目的 建立测定鱼皮胶原蛋白水解液中小分子寡肽相对分子量分布的方法。方法 采用高效凝胶排阻色谱法用3根TSK-GEL G2000SWXL色谱柱(300 mm×7.8 mm，5 μm)串联，室温；流动相为乙腈-0.1%三氟乙酸溶液(15∶85)，流速0.6 mL·min^-1，检测波长220 nm。结果 在相对分子量149~2 490 Da内，寡肽相对分子量的对数与其保留时间具有良好的线性关系(r=0.995 6)；精密度和重复性RSD均<0.1%。结论 本方法简便准确，重复性好，可作为测定小分子寡肽分子量分布的方法。

Abstract:

OBJECTIVE To establish a method for determining the molecular weight distribution of small molecule oligopeptide in fish skin collagen hydrolyzate. METHODS The molecular weight distribution was determined using three TSK-GEL G 2000SWXL columns (300 mm×7.8 mm, 5 μm) in series at room temperature with the mobile phase of acetonitrile-0.1% trifluoracetic acid solution (15∶85) at the flow rate of 0.6 mL·min^-1. The detection wavelength was 220 nm. RESULTS In the molecular weight range of 149-2 490 Da, there was a good linear relationship between the logarithm of molecular weight of oligopeptide and the retention time(r=0.995 6). The RSD of precision and repeatability were both less than 0.1%. CONCLUSION The method is simple, accurate and reproducible for the determination of the molecular weight distribution of small molecule oligopeptide.