| 引用本文: | 巩丽丽,容蓉,杨勇.GC-MS结合保留指数分析侧柏叶挥发成分[J].中国现代应用药学,2011,28(1):48-51. |
| GONG Lili,RONG Rong,YANG Yong.GC-MS Combined with Kovats Retention Indices for Volatile Compounds in Platycladi Cacumen[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(1):48-51. |
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| 摘要: |
| 目的 分析比较不同采集方法制备的侧柏叶中的挥发性成分。方法 分别采用水蒸气蒸馏法和顶空加热提取挥发性成分的方法，对其进行气相色谱-质谱(GC-MS)分析，并结合Kovats保留指数对其主要成分进行定性分析。结果 采用顶空进样技术，可鉴定出侧柏叶中29个挥发性化学成分；采用水蒸气蒸馏法可鉴定出34个挥发性化学成分；两种采样方法进行的GC-MS分析，有23种相同化合物，但相对含量有一定差异。结论 对于侧柏叶，水蒸气蒸馏法提取挥发性成分较完全，顶空直接进样对于易挥发性成分分析则更简便、高效。 |
| 关键词: 侧柏叶 水蒸气蒸馏 顶空进样技术 GC-MS Kovats保留指数 |
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| 基金项目:国家重点基础研究发展计划资助项目(“973”计划)(2007CB512601) |
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| GC-MS Combined with Kovats Retention Indices for Volatile Compounds in Platycladi Cacumen |
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GONG Lili, RONG Rong, YANG Yong
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Shandong University of Traditional Chinese Medicine, Ji′nan 250355, China
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| Abstract: |
| OBJECTIVE To compare the difference of volatile compounds in Platycladi Cacumen treated with different sample acquisition methods. METHODS Using steam distillation and head-space injection respectively, volatile components in Platycladi Cacumen were analyzed by gas chromatography-mass spectrometry(GC-MS) combined with the Kovats retention indices(KI). RESULTS By GC-MS, 29 volatile compounds in Platycladi Cacumen treated with head-space injection were identified, while 34 volatile compounds identified with steam distillation; among the detected compounds, there were 23 mutual compounds with different relative contents. CONCLUSION Volatile compounds can be deprived more by steam distillation, while it can be simple and high effectively with head-space injection. |
| Key words: Platycladi Cacumen steam distillation head-space injection GC-MS Kovats retention indices |
