山楂叶总黄酮对实验性大鼠心肌缺血的作用及其机制研究

Protective Effect and the Mechanisms of Crataegi Folium Flavonoids on Myocardial Ischemia in Rats

关键词:  山楂叶总黄酮  异丙肾上腺素  心肌缺血

Key words:  Crataegi Folium flavonoids  isoproterenol  myocardial ischemia

摘要:

目的 研究山楂叶总黄酮对异丙肾上腺素致大鼠急性心肌缺血的保护作用及其作用机制。方法 取健康SD大鼠，♂，48只，随机分为正常对照组、阳性对照组、模型组及山楂叶总黄酮组(50，100，200 mg·kg^-1)，采用异丙肾上腺素(给药剂量10 mg·kg^-1，每日1次，连续2 d)造成大鼠急性心肌缺血模型，观察急性心肌缺血大鼠心电图的变化，测定血浆的乳酸脱氢酶(LDH)和肌酸激酶(CK)、心肌组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和脂质过氧化物丙二醛(MDA)的含量，并观察心肌缺血大鼠心肌形态学变化。结果 与模型组相比，山楂叶总黄酮(50，100，200 mg·kg^-1)均可有效对抗异丙肾上腺素所致急性大鼠心肌缺血心电图ST段的抬高，降低血浆LDH和CK活力(P<0.05或P<0.01)，增加心肌组织CAT和SOD的活力(P<0.05或P<0.01)，并减少MDA生成(P<0.05或P<0.01)，减轻心肌病理变化。结论 山楂叶总黄酮对异丙肾上腺素致大鼠急性心肌缺血具有一定的保护作用，其机制可能与其抗氧化作用有关。

Abstract:

OBJECTIVE To study the protective effects and the mechanisms of Crataegi Folium flavonoids (CFF) on acute myocardial ischemia induced by isoproterenol in rats. METHODS The 48 healthy male Sprague-Dawley rats were randomly divided into normal control group, positive control group, model group and the group of CFF(50, 100, 200 mg·kg^-1). Myocardial ischemia model of rats were induced by subcutaneous injection of isoproterenol(10 mg·kg^-1 for each time, once a day, and lasted for 2 days). The change of electrocardiogram(ECG) was observed. The levels of lactate dehydrogenase(LDH), cmatine kinase(CK) in blood plasma, the activity of superoxide dismutase(SOD), catalase(CAT), and the content of malondialdehyde(MDA) in myocardial tissue were measured. The changes of myocardial morphology were observed as well. RESULTS Compared with the model group, CFF(50, 100, 200 mg·kg^-1) could effectively fight against ST-segment elevation induced by isoproterenol, notably decrease serum LDH and CK enzymic activity, distinctly increase the levels of CAT and SOD in myocardial tissue(P<0.01 or P<0.05), significantly decrease the content of MDA in myocardial tissue, and relieve morphological changes of myocardial. CONCLUSION CFF has a protective effect on acute myocardial ischemia induced by isoproterenol in rat, the protective mechanisms of CFF may be related to anti-oxidation.