| 引用本文: | 张福成,安静,卢彦芳,孙婷,蒋晔.葡聚糖凝胶色谱法分析注射用阿洛西林钠中的高聚物[J].中国现代应用药学,2011,28(3):257-260. |
| ZHANG Fucheng,AN Jing,LU Yanfang,SUN Ting,JIANG Ye.Determination of High Polymers in Azlocillin Sodium for Injection by Sephadex G-10 Gel Chromatography[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(3):257-260. |
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| 摘要: |
| 目的 建立一个简单分析阿洛西林钠中高聚物的方法。方法 采用SephadexTM G-10色谱柱，以0.1 mol·L-1磷酸盐缓冲液(氢氧化钠调pH 6.8)为流动相A，水为流动相B，流速1.5 mL·min-1，检测波长为254 nm。结果 阿洛西林钠中高聚物在0.001~4.08 mg·mL-1内线性关系良好，检测限为0.5 μg·mL-1。当供试品溶解量为200 mg·mL-1时，本法的高聚物检出能力达到百万分之三。结论 所用方法简便，结果可靠，可用于阿洛西林钠中高聚物的检测。 |
| 关键词: 阿洛西林钠 高聚物 葡聚糖凝胶色谱 |
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| Determination of High Polymers in Azlocillin Sodium for Injection by Sephadex G-10 Gel Chromatography |
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ZHANG Fucheng1, AN Jing2, LU Yanfang2, SUN Ting2, JIANG Ye2
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1.Department of Pharmacy, General Hospital of Air Force, Beijing 100036, China;2.School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China
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| Abstract: |
| OBJECTIVE To develop a simple method for the determination of high polymers in azlocillin sodium for injection. METHODS A SephadexTM G-10 column was used with the mobile phase A of 0.10 mol·L-1 phosphate buffer (adjust to pH 6.8 with NaOH) at a flow rate of 1.5 mL·min-1. And the mobile phase B was pure water, the detection wavelength was 254 nm. RESULTS The calibration curve was linear in the range of 0.001-4.08 mg·mL-1 and the detection limit of high polymers was 0.5 μg·mL-1. The detection potential of high polymers achieved 3 ppm by the method when the dissolved load of azlocillin sodium was 200 mg·mL-1. CONCLUSION The proposed method is simple and reliable. It is applicable for the quality control of azlocillin sodium. |
| Key words: azlocillin sodium high polymers Sephadex G-10 gel chromatograph |
