三七茎基总皂苷的提取及人参皂苷Rg1和Rb1的含量测定

The Extract and the Determination of Ginsenoside Rg1 and Rb1 of Total Saponins of Stem Base of Panax Notognseng

关键词:  液质联用  人参皂苷Rg₁  人参皂苷Rb₁  三七茎基总皂苷

Key words:  LC-MS/MS  ginsenosides Rg₁  ginsenosides Rb₁  total saponins of stem base of Panax notognseng

摘要:

目的 利用LC-MS/MS，建立同时测定三七茎基总皂苷中人参皂苷Rg₁和Rb₁含量的分析方法。方法 色谱柱：BDS HYPERSUL C₁₈柱(150 mm×2.1 mm，5 mm)；流动相：乙腈-水(梯度洗脱)；柱温：30 ℃；质谱条件：采用负离子多反应监测方法(MRM)测试，用于定量分析的对照品离子对分别为：人参皂苷Rg₁ m/z 799.6→475.5；人参皂苷Rb₁ m/z 1 107.9→783.7；内标物紫杉醇m/z 852.5→525.3。结果 人参皂苷Rg₁、人参皂苷Rb₁的线性范围分别为0.173～17.3 mg·mL^-1和0.159～16.0 mg·mL^-1，精密度和准确度等均符合样品分析的要求。结论 该法准确、灵敏、特异性强，适用于三七茎基总皂苷及其制剂中人参皂苷Rg₁、Rb₁浓度的同时测定。

Abstract:

OBJECTIVE To establish an LC-MS/MS method for simultaneous determination of ginsenosides Rg₁ and Rb₁ in total saponins of stem base of Panax notognseng. METHODS BDS HYPERSUL C₁₈ (150 mm×2.1 mm, 5 mm) was used with a mobile phase of acetonitrile-water(gradient elution). The column temperature was 30 ℃. The ginsenosides Rg₁ and Rb₁ were detected by the negative electrospray ionization mode (Rg₁ m/z 799.6→475.5; Rb₁ m/z 1107.9→783.7; paclitaxel m/z 852.5→525.3). RESULTS The calibration curves of ginsenosides Rg₁ and Rb₁ were linear in the range of 0.173-17.3 mg·mL^-1 and 0.159-16.0 mg·mL^-1 respectively. The precision and accuracy were accord with the requirements. CONCLUSION The results show that this method established in the present research is accurate, sensitive and specific. And it is suitable for simultaneous determination of ginsenosides Rg₁ and ginsenosides Rb₁ in total saponins of stem base of Panax notognseng.