| 引用本文: | 刘胜利,黄礼德,郭立强,黄锁义.正交设计法优选穿山龙总皂苷的超声提取工艺[J].中国现代应用药学,2011,28(2):136-139. |
| LIU Shengli,HUANG Lide,GUO Liqiang,HUANG Suoyi.Orthogonal Design for Optimization of Ultrasonic Extracting Procedure for Total Saponins from Dioscoreae Nipponicae Rhizoma[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(2):136-139. |
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| 正交设计法优选穿山龙总皂苷的超声提取工艺 |
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刘胜利1,2,3, 黄礼德1,2,3, 郭立强1,2,3, 黄锁义1,2,3
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1.右江民族医学院，a.临床医学院;2.b.医学检验学院;3.c.药学系，广西 百色 533000
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| 摘要: |
| 目的 确定穿山龙总皂苷的最佳超声提取工艺。方法 采用正交试验设计进行条件优选，通过分光光度法对提取物进行总皂苷含量的测定。结果 检测波长为410 nm，薯蓣皂苷元在0.004~0.020 mg·mL-1内呈良好的线性关系(r=0.999 8)，最佳超声提取工艺为：以水饱和正丁醇为提取剂，料液比1∶30，超声温度70 ℃，超声功率180 W。结论 优选出的超声提取工艺合理、经济、可行。 |
| 关键词: 穿山龙 总皂苷 正交设计 超声提取工艺 |
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| 基金项目:右江民族医学院民族医药协会资助(20100105) |
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| Orthogonal Design for Optimization of Ultrasonic Extracting Procedure for Total Saponins from Dioscoreae Nipponicae Rhizoma |
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LIU Shengli1,2,3, HUANG Lide1,2,3, GUO Liqiang1,2,3, HUANG Suoyi1,2,3
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1.Youjiang Medical College for Nationalities, a.School of Clinical Medicine;2.b.School of Clinical Laboratory Medicine;3.c.Department of Pharmacy, Baise 533000, China
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| Abstract: |
| OBJECTIVE To determine the best ultrasonic extracting procedure for total saponins from Dioscoreae Nipponicae Rhizoma. METHODS Orthogonal experiment was used to optimize the condition. The content of total saponins was determined by spectrophotometric method. RESULTS The wavelength of the maximum absorption was 410 nm. Diosgenin showed a good linear relationship at the range of 0.004-0.020 mg·mL-1(r=0.999 8). The optimum ultrasonic extraction conditions were water-saturated n-butanol as extraction agent, solid-liquid ratio 1∶30, temperature 70 ℃ and ultrasonic power 180 W. CONCLUSION The ultrasonic extracting procedure is reasonable, economical and feasible. |
| Key words: Dioscoreae Nipponicae Rhizoma total saponins orthogonal design the ultrasonic extracting procedure |
