神经节苷脂对大鼠弥漫性轴索损伤后细胞凋亡的影响

Protective Effect of GM-1 on the Apoptosis of Neurons in Rats after Diffuse Axonal Injury

关键词:  弥漫性轴索损伤  神经节苷脂  凋亡蛋白酶激活因子-1

Key words:  diffuse axonal injury  GM-1  apoptotic protease activating factor-1

摘要:

目的 探讨神经节苷脂对大鼠弥漫性轴索损伤后海马CA1区神经元凋亡的影响。方法 Wistar大鼠，♂，192只，随机分为正常对照组、假手术组、脑损伤组和药物干预组。正常对照组不给予任何干预措施；假手术组仅进行头皮切开缝合处理；脑损伤组和药物干预组均制作弥漫性轴索损伤模型，其中药物干预组在制作模型后定期给予神经节苷脂(40 mg·kg^-1·d^-1)。造模3，6，12，24，336 h后，用免疫组化方法检测海马CA1区凋亡蛋白酶激活因子-1(apoptotic protease activating factor-1，Apaf-1)蛋白的表达。结果 脑损伤组和药物干预组大鼠海马CA1区Apaf-1蛋白表达含量均于3 h开始增加，6 h达到高峰，此后逐渐下降。药物干预组大鼠海马CA1区Apaf-1蛋白表达含量在各个时间点均低于脑损伤组，除3 h组外，差异均有统计学意义(P<0.05)。结论 弥漫性轴索损伤可引起大鼠海马CA1区神经元凋亡，神经节苷脂可改善该凋亡的发生发展。

Abstract:

OBJECTIVE To study the protective effect of GM-1 on the apoptosis of neurons in rats after diffuse axonal injury(DAI). METHODS One hundred and ninety two male Wister rats were divided randomly into control group, sham operated group, DAI group and GM-1 treated group. Rats in DAI and GM-1 treated groups were injured by Marmarou method. GM-1 treated group were treated with GM-1(40 mg·kg^-1·d^-1) in peritoneum after injury, other groups were treated with normal saline for comparison at the same time. The expression of apoptotic protease activating factor-1(Apaf-1) was detected by using immunohistochemistry. RESULTS Both the expression of Apaf-1 in DAI rats and GM-1 treated rats began to increase at 3 h after injury and reached the peak at 6 h, then declined. And all of the expression of Apaf-1 in GM-1 treated rats were less than those in DAI rats at 6, 12, 24, 336 h of injury(P<0.05). CONCLUSION DAI can result in the apoptosis of neurons and GM-1 may play a protective role on the apoptosis of neurons in rats with DAI.