HPLC检测中药中非法添加化学降压药物

Detection of Antihypertensive Agents Illegally Added in Traditional Chinese Medicine by HPLC

关键词:  化学降压药物  中药制剂  非法添加  高效液相色谱法

Key words:  antihypertensive agents  traditional Chinese medicine  illegally additives  HPLC

摘要:

目的 为打击中药制剂中非法添加化学降压药物现象，建立了HPLC定性检测中药制剂中可能非法添加的15种化学降压药物。方法 采用Agilent Extend-C₁₈色谱柱(4.6 mm×250 mm，5 μm)，梯度洗脱：流动相A为0.02 mol·L^-1醋酸铵溶液，流动相B为甲醇-乙腈(2∶1)，检测波长为230 nm，流速为1.0 mL·min^-1，柱温为30 ℃。结果 15种化学降压药物有很好的分离度、线性及检测灵敏度。检测限为0.01~13 ng，连续5针进样色谱图中各组分保留时间的RSD均<0.1%。结论 本方法简便灵敏，重复性好，可作为降压类药物的鉴别方法。

Abstract:

OBJECTIVE To hit the phenomenon that antihypertensive agents illegally adulterated in traditional Chinese medicine(TCM) preparations, an HPLC method was established for detection of these fifteen kinds of antihypertensive agents. METHODS The chromatography was performed using an Agilent Extend-C₁₈ column(4.6 mm×250 mm, 5 μm) and gradient elution with the mobile phase consisting of 0.02 mol·L^-1 ammonium acetate solution and methanol-acetonitrile (2∶1) at a flow rate of 1.0 mL·min^-1. The detection wavelength was set at 230 nm. The column temperature was set at 30 ℃. RESULTS Under the condition, there was a good resolution among the fifteen kinds of antihypertensive agents and the detectability was 0.01 ng to 13 ng. Repeated 5 injections yielded relative standard deviation less than 0.1% regarding the retention time of each component. CONSLUSION This method is simple and sensitive with good reproduction quality for identifying antihypertensive agents.