HPLC-ELSD测定心可舒胶囊中三七皂苷R1、人参皂苷Rg1的含量

Determination of Notoginsenoside R1, Ginsenoside Rg1 in Xinkeshu Capsule by HPLC-ELSD

关键词:  心可舒胶囊  高效液相色谱法  蒸发光散射检测器  三七  含量测定

Key words:  Xinkeshu capsule  HPLC  ELSD  Panax notoginseng  content determination

摘要:

目的 建立心可舒胶囊中三七皂苷R₁、人参皂苷Rg1的含量测定方法。方法 以COSMOSIL 5C₁₈-PAQ Packed Column(250 mm×4.6 mm, 5 mm)为色谱柱，以乙腈-水(27∶73)为流动相，流速为1 mL·min^-1，ELSD为检测器，漂移管温度：41 ℃；雾化器(氮气)压力：350 kPa。结果 三七皂苷R₁、人参皂苷Rg1的线性范围分别为0.123 2~6.16，0.327 2~16.36 μg，r值分别为0.999 9，0.999 7；回收率分别为98.7%，99.2%，RSD分别为2.72％，1.54％。结论 该方法简便，准确，灵敏度高，重复性好，可作为心可舒胶囊的含量测定方法。

Abstract:

OBJECTIVE To determine the content of notoginsenoside R₁, ginsenoside Rg1 in Xinkeshu capsule. METHODS The notoginsenoside R₁, ginsenoside Rg1 in Xinkeshu capsule were separated on a COSMOSIL 5C¹⁸-PAQ Packed column(250 mm×4.6 mm, 5 mm). The mobile phase was acetonitrile-water(27∶73) at the flow rate of 1 mL·min^-1. The analytes were detected using ELSD. The drift tube temperature was 41 ℃, and gas press was 350 kPa. RESULTS The responses of notoginsenoside R₁, ginsenoside Rg1 were linear in the ranges of 0.123 2-6.16 μg and 0.327 2-16.36 μg, respectively. The average recoveries were 98.7% and 99.2% with RSD 2.72% and 1.54%, respectively. CONCLUSION The described method is simple and accurate. It can be used for the quality control of Xinkeshu capsule.