五指毛桃水提液对60Coγ射线致小鼠骨髓细胞DNA损伤的防护作用

Protective Role of Aqueous Extract from Fici Hirtae Radix for DNA Damage of Bone Marrow Cells by 60Coγ-Ray in Mice

关键词:  五指毛桃  ⁶⁰Coγ射线  骨髓细胞  DNA损伤  单细胞凝胶电泳

Key words:  Fici Hirtae Radix  ⁶⁰Coγ-rays  bone marrow cells  DNA damage  single-cell gel electrophoresis

摘要:

目的 探讨五指毛桃水提液对⁶⁰Coγ射线所致的小鼠骨髓细胞DNA损伤的防护作用。方法 40只SPF级♂小鼠随机分为5组：阴性对照组给予生理盐水，辐射模型组单纯接受照射，3个五指毛桃剂量组分别给予相当于生药5，10，20 g·kg^-1·d^-1五指毛桃水提液，连续灌胃7 d。除阴性对照组外，所有小鼠接受全身一次性⁶⁰Coγ射线照射，照射剂量为6 Gy。照射24 h后处死小鼠，取股骨，制作骨髓单细胞悬液，用单细胞凝胶电泳实验检测小鼠骨髓细胞DNA损伤情况。结果 辐射模型组小鼠骨髓细胞DNA的尾部DNA百分率(Tail DNA%)和尾矩(Tail Moment)高于阴性对照组(P<0.01)，五指毛桃各剂量组的Tail DNA%和Tail Moment均显著低于辐射模型组(P<0.01)，且随着给药剂量的增加，Tail DNA%和Tail Moment降低。结论 五指毛桃水提液对⁶⁰Coγ射线损伤小鼠骨髓细胞DNA具有一定的防护作用。

Abstract:

OBJECTIVE To investigate the protective role of aqueous extract from Fici Hirtae Radix for bone marrow cells DNA of ⁶⁰Coγ-ray irradiated mice. METHODS Forty SPF male mice were randomized into 5 groups. Negative control group was given normal saline, irradiated group was radiation model group and 3 Fici Hirtae Radix dose groups were orally given aqueous extracts from Fici Hirtae Radix of 5, 10, 20 g·kg^-1·d^-1 for 7 consecutive days. Except the negative control group, all mice received 6 Gy irradiation of whole body. After 24 h of irradiation, mice were sacrificed on femur. Single cell suspension was produced. DNA damage was detected using single cell gel electrophoresis experiment. RESULTS The results showed that Tail DNA% and Tail Moment of radiation model mice were higher than negative control group (P<0.01). Tail DNA% and Tail Moment of all Fici Hirtae Radix dose groups were significantly lower than radiation model group(P<0.01). As the dose increased, Tail DNA% and Tail Moment reduced. CONCLUSION Aqueous extracts from Fici Hirtae Radix have good protective effect on ⁶⁰Coγ-organization of bone marrow cells DNA damage in mice.