| 引用本文: | 张欢,唐辉,贾蔓箐,杨四涛,吴旭东.HPLC测定棉油皂脚中棉酚含量[J].中国现代应用药学,2011,28(2):162-164. |
| ZHANG Huan,TANG Hui,JIA Manqing,YANG Sitao,WU Xudong.Determine of Gossypol in Cottonseed Soapstock by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(2):162-164. |
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| 摘要: |
| 目的 建立高效液相色谱法测定棉油皂脚中总棉酚(TGP)与游离棉酚(FGP)含量的方法。方法 采用Waters Bondapak C18 Column(3.9 mm×150 mm，4 μm)；流动相为乙腈-0.7%磷酸水溶液(80∶20)；流速为1.0 mL·min-1；检测波长为238 nm；柱温为25 ℃。结果 棉酚在10~60 μg·mL-1(r=0.999 4)内线性关系良好，平均加样回收率为99.03%，RSD=1.72%(n=3)。结论 本法简便、快速、准确、重复性高，可用于对棉油皂脚中棉酚的含量测定。 |
| 关键词: 高效液相色谱法 棉油皂脚 棉酚 |
| DOI： |
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| 基金项目:新疆建设兵团博士基金项目(2009JC14)；国家教育部科学技术研究重点项目(205185) |
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| Determine of Gossypol in Cottonseed Soapstock by HPLC |
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ZHANG Huan, TANG Hui, JIA Manqing, YANG Sitao, WU Xudong
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Key Laboratory of Xinjiang Phytomedicine Resources, Pharmacy College of Shihezi University, Shihezi 832002, China
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| Abstract: |
| OBJECTIVE To determine the content of total gossypol and liberation gossypol by HPLC. METHODS The analysis was performed on a Waters Bondapak C18 Column(3.9 mm×150 mm, 4 μm) at 25 ℃ with acetonitrile-0.7% phosphoric acid water solution(80∶20) as the mobile phase and the flow rate of 1.0 mL·min-1. The detection wavelength was set at 238 nm. RESULTS Gossypol have good linearity in the range of 10-60 μg·mL-1(r=0.999 4). The average recovery was 99.03%, RSD=1.72%(n=3). CONCLUSION The developed method is convenient, rapid and accurate with high repeatability, which is suitable to assay the quality of gossypol. |
| Key words: HPLC cottonseed soapstock gossypol |
