蝎毒纤溶活性肽对大鼠局灶性脑缺血再灌注损伤保护作用研究

Effect of Scorpion Venom Fibrinolytic Active Peptides on Cerebral Ischemia/Reperfusion Injury in Rats

关键词:  蝎毒纤溶活性肽  脑缺血再灌注  炎性细胞因子

Key words:  scorpion venom fibrinolytic active peptides  cerebral ischemia/reperfusion injury  inflammatory cell factors

摘要:

目的 研究蝎毒纤溶活性肽对大鼠脑缺血再灌注损伤的保护作用。方法 采用大鼠右侧大脑中动脉阻塞(MCAO)2 h，再灌注24 h模型，造模前，蝎毒纤溶活性肽组分别静脉注射蝎毒纤溶活性肽0.1，0.2，0.4 mg·kg^-1，尼莫地平组注射尼莫地平0.7 mg·kg^-1，1次·d^-1，连续7 d。分别以Longa￠s法评定神经功能、红四氮唑(TTC)染色测定脑梗死体积及放射免疫技术检测血清炎性细胞因子TNF-a、IL-6以及IL-8等的变化。结果 与模型组比较，蝎毒纤溶活性肽能明显改善大鼠MCAO后神经功能症状，缩小梗塞体积(P<0.05)，并抑制血清炎性细胞因子TNF-a、IL-6及IL-8的表达(P<0.05)。结论 蝎毒纤溶活性肽对大鼠脑缺血再灌注损伤有保护作用，其机制可能与抑制炎性细胞因子表达有关。

Abstract:

OBJECTIVE To investigate the protective effect of scorpion venom fibrinolytic active peptide(SVFAPs) on cerebral ischemia/reperfusion injury in rats. METHODS　In this study, cerebral ischemia-reperfusion injury model was made by 2 hours of middle cerebral artery occlusion (MCAO) followed by 24 hours of reperfusion in rats￠ right cerebral. Rats were pretreated with SVFAPs 0.1, 0.2, 0.4 mg·kg^-1, nimodipine 0.7 mg·kg^-1 once a day for 7 days and then subjected to MCAO. We examined neurological infarction size by 2, 3, 5-triphenyltetrazolium chloride (TTC) staining technique, the neurological deficit scores grading on a scale of 0-5, and the contents of IL-6, TNF-α and IL-8 in the serum. RESULTS SVFAPs could obviously decrease infarction volumes, neurological deficit scores (P<0.05). And SVFAPs inhibited the expression of IL-6, TNF-α and IL-8(P<0.05). CONCLUSION SVFAPs may play a neural protection effect by inhibiting the expression of IL-6, TNF-α and IL-8.