黄芩苷对香烟烟雾提取物诱导的细胞损伤保护作用研究

Effect of Baicalin on Cell Damage Induced by Cigarette Smoke Extract

关键词:  黄芩苷  香烟烟雾提取物  细胞凋亡  活性氧  DNA损伤

Key words:  baicalin  cigarette smoke extract  apoptosis  reactive oxygen species  DNA damage

摘要:

目的 研究黄芩苷对香烟烟雾提取物(cigarette smoke extract，CSE)诱导的人肺泡上皮细胞损伤的影响，并初步探讨其机制。方法 制备CSE，用不同浓度黄芩苷对人肺泡上皮细胞A549进行预处理，再用CSE 刺激细胞。用MTT法检测细胞存活率，流式细胞术测定细胞凋亡率，彗星实验观察DNA损伤情况，荧光法测定细胞内活性氧(reactive oxygen species，ROS) 含量。结果 随着CSE浓度的增加和作用时间的延长，细胞存活率下降，各组之间有统计学差异(P<0.05)；黄芩苷能减少CSE诱导的细胞存活率下降，抑制CSE诱导的细胞内ROS的产生，并有剂量依赖关系(P<0.05或P<0.01)；黄芩苷+CSE组细胞的彗星尾长、尾部DNA含量、尾距、Olive尾距均小于CSE组(P<0.05)，并且黄芩苷可以减少CSE导致的细胞凋亡。结论 黄芩苷可以拮抗CSE对细胞的损伤，提高细胞的存活率，降低凋亡率，其原因可能与黄芩苷能降低细胞ROS含量，减少DNA损伤有关。

Abstract:

OBJECTIVE To investigate the effect of baicalin on A549 cells exposed to cigarette smoke extract (CSE). METHODS Cultured human lung adenocarcinoma A549 cells. Mainstream smoke was collected by using dimerhyl sulfoxide as absorbent. A549 cells were incubated with different concentrations of baicalin and CSE. Cell viability was evaluated by MTT assay. Cell apoptosis was detected by flow cytometry annexin V-FTTC/PI staining assay. Comet assay was used to detected DNA damage. The contents of reactive oxygen species (ROS) was examined by fluorescence method. RESULTS MTT assay results showed that CSE decrease the viability of A549 cells in time- and concentration-dependent manners(P<0.05). Baicalin inhibited the decrease of viability induced by CSE(P<0.05 or P<0.01), decreased the contents of ROS in A549 cells (P<0.01). The comet assay indicated that baicalin inhibited the DNA damage induced by CSE on A549 cells, tail length, tail DNA%, TM, OTM were lower than CSE group(P<0.05). And apoptosis rate of cells injured by CSE was decreased by baicalin. CONCLUSION Bacalin can inhibit the injury induced by CSE, increase viability, decrease apoptosis. The results may be related to the decrease of ROS and a lower DNA damage rate caused by baicalin.