超声法与闪式提取法提取龙胆中龙胆苦苷的工艺对比研究

Comparison of Ultrasonic Method and Smashing Tissue Extraction Process of Gentiopicroside from Gentianae Radix et Rhizoma

关键词:  超声法  闪式提取法  龙胆  龙胆苦苷  高效液相色谱法

Key words:  ultrasonic method  smashing tissue extraction process  Gentianae Radix et Rhizoma  gentiopicroside  HPLC

摘要:

目的 对比研究超声法与闪式提取法提取龙胆中龙胆苦苷的工艺。方法 采用高效液相色谱法测定龙胆中龙胆苦苷的含量，超声法以乙醇浓度、提取时间、超声功率、料液比为考察因素，闪式提取法以乙醇浓度、料液比、提取时间为考察因素，以龙胆苦苷提取率为考察指标，采用L₉(3⁴)正交试验优选最佳提取工艺条件。结果 与超声法相比，闪式提取法效率更高，其最佳提取工艺为提取溶剂50%乙醇，料液比1∶60，提取时间3 min。结论 本法工艺简单、经济、提取率高，可为中药龙胆的开发利用提供参考。

Abstract:

OBJECTIVE To compare the ultrasonic extraction and smashing tissue extraction process of gentiopicroside from Gentianae Radix et Rhizoma. METHODS An HPLC method was applied to determine the content of gentiopicroside from Gentianae Radix et Rhizoma. The best extraction technology was optimized by L₉(3⁴) orthogonal test with the concentration of ethanol, extraction time, ultrasonic power, material-liquid ratio as factors for ultrasonic extraction and with the concentration of ethanol, material-liquid ratio, extraction time as factors for smashing tissue extraction process, the extraction rate of gentiopicroside was chosen as index. RESULTS Smashing tissue extraction process was superior to ultrasonic extraction, optimal extraction technology were as follows: extraction solvent of 50% ethanol, the material-liquid ratio of 1∶60, extracting time of 3 min. CONCLUSION The smashing tissue extraction process is simple and economical with high extraction rate and can provide parameters for exploitation and utilization of Gentianae Radix et Rhizoma.