蒙药材飞廉的定性及定量研究

Qualitative and Quantitative Study of Mongolian Medicine Carduus Crispus

关键词:  飞廉  绿原酸  薄层色谱法  高效液相色谱法

Key words:  Carduus crispus  chlorogenic acid  TLC  HPLC

摘要:

目的 建立飞廉药材的定性及定量方法。方法 分别采用化学法和薄层色谱法对飞廉药材中的黄酮类成分和绿原酸进行定性鉴别，并采用高效液相色谱法测定绿原酸的含量。色谱柱为Hypersil ODS2柱(4.6 mm×250 mm，5 μm)；流动相为甲醇-0.5%醋酸(15∶85)；流速为1.0 mL·min^-1；柱温为25 ℃；检测波长为325 nm。结果 定性鉴别试验特征明显，绿原酸在0.088~1.76 μg内呈良好的线性关系，平均回收率为100.3%(RSD为1.52%)。结论 该方法简便、快速，重复性好，准确度高，可有效控制飞廉药材的质量。

Abstract:

OBJECTIVE To establish a method for the qualitative and quantitative determination of Carduus crispus. METHODS Flavonoids and chlorogenic acid were identified by chemical method and TCL respectively, and the chlorogenic acid was determined by HPLC. The analysis of chlorogenic acid was carried on a Hypersil ODS2 column(4.6 mm×250 mm, 5 μm) using methanol-0.5% acetic acid(15∶85) as mobile phase at a flow rate of 1.0 mL·min^-1. The column temperature was set at 25 ℃, and detecting wavelength was 325 nm. RESULTS The obvious characteristics were observed in qualitative identification test. There was a good linear relationship over the range of 0.088-1.76 μg for chlorogenic acid. The average recovery was 100.3% with RSD of 1.52%. CONCLUSION The developed method is simple, rapid, reproducible and accurate. It can be used for the quality control of Carduus crispus.