重庆产泽漆中总酚酸与没食子酸的含量分析

Content Analysis on Total Phenolic Acid and Gallic Acid in Ephorbia Helioscopia L. in Chongqing

关键词:  比色法  高效液相色谱法  泽漆  总酚酸  没食子酸

Key words:  colorimetry  HPLC  Ephorbia helioscopia L.  total phenolic acid  gallic acid

摘要:

目的 建立泽漆中总酚酸与没食子酸含量分析的方法，研究重庆产泽漆中总酚酸与没食子酸的含量。方法 采用FeCl3-K3[Fe(CN)6]比色法测定泽漆中总酚酸的含量，以没食子酸为对照品，检测波长为720 nm；采用HPLC测定泽漆中没食子酸的含量，色谱柱为Hypersil C₁₈柱(250 mm×4.6 mm，5 μm)，流动相为乙腈-0.1%磷酸(10∶90)，流速为1.0 mL·min^-1，检测波长为270 nm。结果 分光光度法测定泽漆总酚酸在6.75～67.5 μg内线性关系良好(r=0.999 5)，平均加样回收率为97.41%。HPLC测定没食子酸在0.52~5.2 μg内线性关系良好(r=0.999 8)，平均加样回收率为98.02%。重庆不同产地泽漆中总酚酸与没食子酸的含量均存在一定差异，总酚酸含量为5.327～5.464 mg·g^-1，没食子酸含量为0.995~1.087 mg·g^-1。结论 本方法适用于泽漆中酚酸类物质的质量分析，本研究可以为泽漆的质量控制提供参考。

Abstract:

OBJECTIVE To establish a method to determine and study the content of total phenolic acid and gallic acid in Ephorbia helioscopia L. in Chongqing. METHODS The content of total phenolic acid was determined by ferric chloride-ferricyanatum Kalium colorimetric method. In this method, gallic acid was used as the reference substance and UV detection wavelength was 720 nm. The content of gallic acid in Ephorbia helioscopia L. was determined by HPLC with an Hypersil C₁₈ column(250 mm×4.6 mm, 5 μm). The chromatographic conditions included the mobile phase consisting of acetonitrile-0.1% phosphoric acid water solution(10∶90) at a flow rate of 1.0 mL·min^-1, and UV detection wavelength was 270 nm. RESULTS A good linear relationship at the range of 6.75-67.5 μg(r=0.999 5) was obtained while determining total phenolic acid and the average recovery was 97.41%. Also a good linear relationship at the range of 0.52-5.2 μg(r=0.999 8) was obtained while determining total gallic acid and the average recovery was 98.02%. The content of total phenolic acid and gallic acid in various populations were different. The content of total phenolic acid in Ephorbia helioscopia L. was 5.327-5.464 mg·g-1. The content of gallic acid in Ephorbia helioscopia L. was 0.995-1.087 mg·g_-1. CONCLUSION The mothod is suitable for quality control of Ephorbia helioscopia L.. The experiment can be reference to the quality appraisal for Ephorbia helioscopia L..