HPLC同时测定飞扬草中没食子酸和槲皮苷的含量

Determination of Gallic Acid and Quercitrin in Euphorbia Hirta L. by HPLC

关键词:  高效液相色谱法  飞扬草  槲皮苷  没食子酸  含量测定

Key words:  HPLC  Euphorbia hirta L.  quercitrin  gallic acid  content determination

摘要:

目的 建立飞扬草中没食子酸和槲皮苷的含量测定方法。方法 采用高效液相色谱法，ZORBAX SB-Aq(4.6 mm×250 mm，5 μm)色谱柱，柱温 30 ℃；流动相为甲醇-乙腈-0.2%磷酸溶液，采用梯度方式洗脱；流速1.0 mL·min^-1；检测波长随时间变换。结果 没食子酸和槲皮苷在2.328~58.20 mg·mL^-1和6.804~170.1 mg·mL^-1内呈良好的线性关系，r分别为0.999 5和0.999 8；平均回收率为100.26%和96.29%，RSD分别为0.64%和0.69%(n=9)。结论 该方法快速，简便，准确，为飞扬草药材及其制剂的质量控制提供参考。

Abstract:

OBJECTIVE To establish an HPLC method for the determination of gallic acid and quercitrin in Euphorbia hirta L. METHODS Using ZORBAX SB-Aq(4.6 mm×250 mm, 5 μm)column, at 30 ℃; methanol-acetonitrile-0.2% phosphoric acid solution was used as the mobile phase, with gradient elution. The flow rate was 1.0 mL·min^-1. The detection wavelength was changed by time. RESULTS The gallic acid and quercitrin had a good linearity relationship between 2.328-58.20 mg·mL^-1 and 6.804-170.1 mg·mL^-1, with r=0.999 5 and 0.999 8. The average recovery rate were 100.26% and 96.29%, with RSD of 0.64% and 0.69%(n=9). CONCLUSION The method is simple, rapid and accurate, suitable for the quality control of Euphorbia hirta L.