膨润土对阿司匹林致小鼠胃黏膜损伤模型的保护作用

Protective Effect of Bentonite on Gastric Mucosal Injury Model Induced by Aspirin

关键词:  膨润土  阿司匹林  胃黏膜

Key words:  bentonite  aspirin  gastric mucous

摘要:

目的 探讨膨润土对胃黏膜损伤的保护作用，为开发该药物制剂奠定基础。方法 采用阿司匹林诱发小鼠胃黏膜损伤模型，观察胃黏膜损伤程度并计算溃疡指数；做胃组织病理学检查；测定血清中丙二醛(MDA)的含量、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力；并测定胃内容物pH值；应用阿利斯蓝染色法测定胃壁结合黏液量。结果 通过病理检查和生化指标检测可知，当膨润土的剂量为50 mg·kg^-1时，就可对模型小鼠的胃黏膜产生一定的保护作用，当膨润土剂量达200~300 mg·kg^-1时，可明显改善胃黏膜损伤，抑制胃黏膜MDA的生成，恢复SOD和GSH-Px活性，且可使胃黏膜的黏液量明显增加，但对胃内容物的pH值无明显的影响。结论 膨润土剂量为200~300 mg·kg^-1时，对阿司匹林致小鼠胃黏膜损伤模型具有明显的保护作用。

Abstract:

OBJECTIVE To research and assess bentonite on the protection of gastric mucosal injury to lay the foundation for the study of its pharmaceutical preparations. METHODS Gastric mucosal injury was induced by aspirin, and ulcer index was observed and calculated. Through gastric histopathology, gastric mucosal MDA content and SOD, GSH-Px activity in serum were measured. pH value of stomach contents was assayed. Gastric barrier mucus was quantitatively determined by means of Alcian blue to stain acidic mucus. RESULTS The pathological examination and biochemical tests presented that when bentonite dose was 50 mg·kg^-1, it had a protective effect on the gastric mucosa of the mice; when the bentonite dose was 200-300 mg·kg^-1, it could significantly improve gastric mucosal damage, inhibit MDA of gastric mucosal, restore the activity of SOD and GSH-Px, and significantly increase the gastric mucus content, but the pH value of stomach contents had no significant effect. CONCLUSION When the dose of bentonite is 200-300 mg·kg^-1, it has an obvious protective effect on gastric mucosal injury model induced by aspirin.