花椒多酚类化合物对衰老小鼠记忆障碍的改善作用

Effect of Polyphenol Extract from Zanthoxylum Bungeanum Maxim. on Learning and Memory Abilities in Senile Mice Induced by D-Galactose

关键词:  花椒  多酚类化合物  D-半乳糖  学习记忆  超氧化物歧化酶

Key words:  Zanthoxylum bungeanum maxim.  polyphenols  D-galactose  learning and memory  SOD

摘要:

目的 探讨花椒多酚类化合物总提取物(ZPPC)对D-半乳糖致衰老模型小鼠学习记忆能力及抗氧化能力的影响。方法 模型组，ZPPC低、中、高剂量组和阳性对照组皮下注射10%D-半乳糖6周建立衰老小鼠模型，ZPPC低、中、高剂量组同时灌胃给予ZPPC，正常对照组和模型组给予等量的生理盐水，阳性对照组同时灌胃给予维生素E。用Morris水迷宫实验检测小鼠的学习记忆能力，同时检测小鼠脑组织超氧化物歧化酶(SOD)活力以及丙二醛(MDA)含量。结果 与正常对照组比较，模型组小鼠学习记忆能力降低，脑组织SOD活力下降，MDA含量增加，均有统计学差异(P<0.01)。灌胃给予高中低不同剂量的ZPPC后，小鼠上述变化明显改善，与模型组比较有统计学差异(P<0.05或P<0.01)。结论 ZPPC可改善D-半乳糖衰老模型小鼠的学习记忆能力，其作用机制可能与ZPPC具有抗氧化能力有关。

Abstract:

OBJECTIVE To observe the effects of polyphenol extract from Zanthoxylum bungeanum Maxim. (ZPPC) on learning and memory function and in senile model mice induced by D-galactose. METHDDS The senile model mice were established by D-galactose hypodermic injection for 6 weeks and given ZPPC in therapeutic group and the same normal saline in the normal control group and model group at the same time. The mice of positive control group were given with vitamin E. The learning and memory ability of all mice were tested with Morris water maze. Then the mice were killed and biochemically assayed of superoxide dismutase (SOD) and malondialdehyde (MDA) in the brain were detected. RESULTS Compared with normal control group, the learning and memory abilities in model group were lowered (P<0.01), SOD activity in the brain decreased (P<0.01) and the MDA content increased (P<0.01). And after given ZPPC, all the abilities were significantly improved (P<0.05 or P<0.01). CONCLUSION ZPPC can improve the learning and memory abilities which may be related to the modulation of the anti-oxidative ability.