| 引用本文: | 刘彩红，王爱玲，李玉琴，李珂，贾宝秀.款冬花多糖抗氧化能力测定[J].中国现代应用药学,2011,28(10):886-889. |
| LIU Caihong, WANG Ailing, LI Yuqin, LI Ke, JIA Baoxiu.Determination of Antioxidation of Polysaccharides in Tussilago Farfara[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(10):886-889. |
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| 摘要: |
| 目的 用流动注射化学发光法探讨中药款冬花多糖的体外抗氧化作用。方法 将款冬花多糖提取液加入3种化学发光体系，测量其发光强度，根据系统化学发光被抑制的程度评价款冬花对活性氧自由基的清除能力，并以抗坏血酸(Vc)为阳性对照。结果 款冬花多糖对·OH有很好的清除作用，对O2-·、H2O2也有一定的清除作用，且在0.25~4.4 mg·L-1内清除作用随浓度的增加而增强；对自由基的清除能力大小：·OH>H2O2>O2-·。结论 在一定浓度范围内，款冬花多糖具有一定的抗氧化活性。 |
| 关键词: 流动注射化学发光 款冬花多糖 抗氧化 |
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| 基金项目:山东省教育厅基金资助项目(J09LF30) |
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| Determination of Antioxidation of Polysaccharides in Tussilago Farfara |
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LIU Caihong, WANG Ailing, LI Yuqin, LI Ke, JIA Baoxiu
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Pharmacy Department of Taishan Medical University, Taian 271000, China
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| Abstract: |
| OBJECTIVE To determine the scavenging ability on ROS free radical of polysaccharides in Tussilago farfara in vitro by flow injection chemiluminescence. METHODS The determination was based on the inhibition effect of Tussilago farfara polysaccharides extraction in three chemiluminescence system, and vitamin C was used as positive control. RESULTS It was showed that Tussilago farfara original liquid could inhibit and clear the ROS such as O2-·, ·OH and H2O2 effectively, and the efficiency was related to the concentration of polysaccharides in Tussilago farfara in the concentration range from 0.25 to 4.4 mg·L-1. The scavenging abilities of polysaccharides in Tussilago farfara on ROS was ·OH>H2O2>O2-·. CONCLUSION The polysaccharides of Tussilago farfara have an antioxidation effect within a range of certain concentration in vitro. |
| Key words: flow injection chemiluminescence polysaccharides of Tussilago farfara antioxidation |
