PTD-SOD对Na2S2O4诱导的SH-SY5Y细胞损伤的保护和修复作用研究

Protective Effects of PTD-SOD Fusion Protein on SH-SY5Y Cell Induced by Sodium Dithionite Sodium Hydrosulfite

关键词:  融合蛋白  自由基  缺氧损伤  人神经母细胞瘤细胞  抗氧化

Key words:  fusion protein  free radical  hypoxia damage  SH-SY5Y  antioxidant ability

摘要:

目的 研究PTD-SOD对Na₂S₂O₄诱导的SH-SY5Y细胞损伤的保护和修复作用。方法 建立Na₂S₂O₄诱导的SH-SY5Y细胞缺氧损伤模型，采用MTT法检测融合蛋白PTD-SOD对细胞存活率的影响，LDH释放分析检测PTD-SOD对细胞损伤后的保护作用及损伤后细胞内氧化与抗氧化水平的变化。结果 对Na₂S₂O₄诱导的SH-SY5Y细胞缺氧损伤，MTT活性检测、LDH分析及氧化与抗氧化水平分析都表明PTD-SOD能明显提高细胞存活率和细胞内抗氧化能力。结论 PTD-SOD融合蛋白能保护Na₂S₂O₄诱导缺氧损伤的SH-SY5Y细胞，具有抑制细胞死亡，改善损伤细胞内抗氧化能力。

Abstract:

OBJECTIVE To investigate the protection mechanism of fusion protein PTD-SOD on SH-SY5Y cell against toxicity induced by sodium dithionite sodium hydrosulfite. METHODS Establish the hypoxia damage model of SH-SY5Y cell induced by Na₂S₂O₄. MTT assay was used to measure the survival of SH-SY5Y cell. LDH, SOD and MDA were measured by spectrophotometric method to analysis the protective effect of PTD-SOD on SH-SY5Y cell and the change of the cell oxidation and antioxidant levels after the hypoxia damage. RESULTS MTT assay demonstrated that PTD-SOD could promote significantly the survival of the cell. LDH release assay and SOD and MDA assay demonstrated that PTD-SOD could promote the levels of antioxidant against the hypoxia damage. CONCLUSION The fusion protein PTD-SOD protects SH-SY5Y cell from death after injury induced by Na₂S₂O₄ and promotes antioxidant ability in cell.