HPLC测定转化糖注射液中果糖、葡萄糖含量

Determination of Fructose and Glucose in Invert Sugar Injection by HPLC

关键词:  转化糖注射液  高效液相色谱法  果糖  葡萄糖  含量测定

Key words:  invert sugar injection  HPLC  fructose  glucose  content determination

摘要:

目的 建立转化糖注射液中果糖、葡萄糖的含量测定方法。方法 色谱柱为Hamilton HC-75H(305 mm×7.8 mm，9 μm)，流动相为0.04 mol·L^-1磷酸，流速为0.5 mL·min^-1，检测波长为200 nm，柱温为50 ℃。结果 果糖和葡萄糖的检测限分别为0.28，0.52 μg；线性范围分别为1.27~3.81 mg·mL^-1(r=0.999 4)、1.26~3.76 mg·mL^-1(r=0.999 2)；加样回收率分别为100.8%，100.4%。结论 本法适用于转化糖注射液中果糖、葡萄糖的含量测定。

Abstract:

OBJECTIVE To establish a method for the determination of fructose and glucose in invert sugar injection. METHODS Hamilton HC-75H(305 mm×7.8 mm, 9 μm) was used. The mobile phase was 0.04 mol·L^-1 phosphoric acid at a flow rate of 0.5 mL·min^-1. The determination wavelength was 200 nm. The temperature of the column was 50 ℃. RESULTS To fructose and glucose, the limit of detection was 0.28 μg and 0.52 μg, the linear range was 1.27-3.81 mg·mL^-1(r=0.999 4) and 1.26-3.76 mg·mL^-1(r=0.999 2), the spiked recovery was 100.8% and 100.4%, respectively. CONCLUSION The method is suitable for the determination of fructose and glucose in invert sugar injection.