| 引用本文: | 夏勇，张双凤，于村，上官小来，黄百芬，邹艳.转乙肝抗原蛋白基因苜蓿毒理学安全性的研究[J].中国现代应用药学,2011,28(8):696-701. |
| XIA Yong, ZHANG Shuangfeng, YU Cun, SHANGGUAN Xiaolai, HUANG Baifen, ZOU Yan.Study on Toxicology Safety of Alfalfa Transformated with HBV Antigen Protein Gene[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(8):696-701. |
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| 摘要: |
| 目的 对转乙肝抗原蛋白基因苜蓿进行安全性评价试验研究，为其进一步研发提供依据。方法 采用急性毒性试验、遗传毒性试验(包括V79细胞基因突变试验、Ames试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验)对转乙肝抗原蛋白基因苜蓿进行系统的安全性研究。结果 转乙肝抗原蛋白基因苜蓿最大耐受剂量>10.0 g·kg-1，属实际无毒级。V79细胞基因突变试验、Ames试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验结果均为阴性。结论 转乙肝抗原蛋白基因苜蓿属实际无毒类物质，在本试验条件下无致基因突变和染色体畸变作用。 |
| 关键词: 转乙肝抗原蛋白基因苜蓿 安全性 |
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| 基金项目:浙江省医药卫生科学研究基金(2009B039) |
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| Study on Toxicology Safety of Alfalfa Transformated with HBV Antigen Protein Gene |
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XIA Yong, ZHANG Shuangfeng, YU Cun, SHANGGUAN Xiaolai, HUANG Baifen, ZOU Yan1,2
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1.Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China;2.Zhejiang Chemical Industry Research Institute, Hangzhou 310023, China
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| Abstract: |
| OBJECTIVE To evaluate the safety of alfalfa transformated with HBV antigen protein gene (ATHAPG) for its further research. METHODS Systematic safety was evaluated by acute toxicity and genotoxicity experiments including V79/HGPRT gene mutation test, mice bone marrow micro-nuclear test, Ames test and mice sperm abnormality test. RESULTS The maximal tolerance dose was higher than 10.0 g·kg-1, all genetic toxicity tests were negative. CONCLUSION ATHAPG is an actually non-toxic substance. ATHAPG has no mutagenic effects under the experimental conditions. |
| Key words: alfalfa transformated with HBV antigen protein gene safety |
