不同方法提取薰衣草挥发油主要成分的GC测定

Content Determination of the Essential Oil from Lavender with Different Extraction Methods by GC

关键词:  气相色谱法  薰衣草挥发油  芳樟醇  乙酸芳樟酯  含量测定

Key words:  GC  lavender oil  linalool  linalyl acetate  content determination

摘要:

目的 GC测定不同方法提取薰衣草挥发油中芳樟醇和乙酸芳樟酯的含量。方法 采用毛细管气相色谱法，以间二硝基苯为内标，采用DB-5弹性石英毛细管柱(30 m×0.25 mm，0.25 μm)，FID检测器；进样口温度为：250 ℃，分流比20∶1，流量：1.0 mL·min^-1，检测器温度：220 ℃；程序升温：110 ℃保持3 min，以10 ℃·min^-1升至130 ℃保持4 min，然后以20 ℃·min^-1升至250 ℃保持1 min。结果 在该色谱条件下，芳樟醇、乙酸芳樟酯及内标物间二硝基苯均得到良好的分离；测得超临界CO₂萃取的精油中芳樟醇的含量为38.02%，乙酸芳樟酯的含量为34.24%，而通过水蒸气蒸馏法提取的精油中芳樟醇的含量为59.09%，乙酸芳樟酯的含量为18.14%。结论 本方法简便、准确、重复性好，可用于不同提取方法提取的薰衣草挥发油的质量控制。

Abstract:

OBJECTIVE To determine the linalyl acetate and linalool content in the essential oil from lavender with different extraction methods by GC. METHODS The capillary GC system consisted of a DB-5 capillary column(30 m×0.25 mm, 0.25 μm), and meta-dinitro-benzent was used as an internal standard. The detector was FID; inlet temperature was 230 ℃; split ratio was 20∶1; the flow rate was 1.0 mL·min^-1; the detector temperature was 250 ℃. The incept temperature programmed was 110 ℃ and kept for 3 min; programmed from 110 ℃ to 130 ℃ at 10 ℃·min^-1 and kept for 4 min, and then progammed to 250 ℃ at 20 ℃·min^-1 and kept for 1 min. RESULTS Linalool, linalyl acetate and the internal standard were separated in the chromatographic condition. The linalool content from the supercritical CO₂ extract in the volatile oil was 38.02%, the linalyl acetate content was 34.24%, the linalool content from the steam distillation extract in the volatile oil was 59.09%, the linalyl acetate content was 18.14%. CONCLUSION The method is accurate, selectivity, and can be used as quality control for the lavender oil extracted with different methods.