| 引用本文: | 方唯意;唐小卿;杨慧玲;曹守春;曹建国.重组人内皮抑素在大肠杆菌中表达及其生物学活性鉴定[J].中国现代应用药学,2003,(2):85-88. |
| FANG Wei-yi ,TANG Xiao-qing, YANG Hui-ling, CAO Shou-chun, CAO Jian-guo.Expression of recombinant human endostatin in E.coli and identification of its biological activity[J].Chin J Mod Appl Pharm(中国现代应用药学),2003,(2):85-88. |
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| 摘要: |
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| 关键词: 人内皮抑素 逆转录聚合酶链反应 原核表达系统 鸡胚尿囊膜 |
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| 基金项目:湖南省教委科研基金项目No :99C202;;湖南省卫生厅重点课题编号:4-01-WJ-2001-Y055 |
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| Expression of recombinant human endostatin in E.coli and identification of its biological activity |
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FANG Wei-yi ,TANG Xiao-qing, YANG Hui-ling, CAO Shou-chun, CAO Jian-guo
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| Abstract: |
| OBJECTIVE To express recombinant human endostatin in E.coli and identify its biological activity. METHOD Endostatin cDNA was amplificated by RT PCR from human fresh fetal liver. The synthesized cDNA fragment was cloned into pGEM-T Easy vector. After i |
| Key words: human endostatin RT-PCR prokaryotic expression the chicken chorio allantoicmembrane(CAM) |
