引用本文: | 周淡宜;徐水祥;唐靓.猪血红细胞膜中鞘磷脂降解成神经酰胺的实验研究[J].中国现代应用药学,2004,(3):169-170. |
| ZHOU Dan-yi, XU Shui-xiang, TANG Liang.Study on the hydrolyzation of the sphingomyelin from pig erythrocyte membrane to ceramide[J].Chin J Mod Appl Pharm(中国现代应用药学),2004,(3):169-170. |
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摘要: |
目的 从红细胞膜中提取鞘磷脂并降解成神经酰胺。方法 分离猪血细胞,用溶血,脱水,干燥等方法提取鞘磷脂,并用磷脂酶C降解鞘磷脂成神经酰胺。结果 用此法可提取到红细胞膜中的鞘磷脂,并最后获得神经酰胺。结论 用此法可将红细胞膜中的鞘磷脂降解成神经酰胺。 |
关键词: 红细胞膜 鞘磷脂 提取 磷脂酶C 神经酰胺 |
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Study on the hydrolyzation of the sphingomyelin from pig erythrocyte membrane to ceramide |
ZHOU Dan-yi, XU Shui-xiang, TANG Liang
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Abstract: |
OBJECTIVE To extract the sphingomyelin from erythrocyte membrane and hydrolyze it to ceramide. METHOD Sphingomyelin was prepared through the process of separating erythrocyte from blood,erythrocytolysis,dehydration,dry,et al,and then hydrolyzed to cera |
Key words: erythrocyte membrane sphingomyelin extraction phospholipase C ceramide |