紫金砂总香豆素的超声辅助提取工艺研究

Study on Ultra Sonic-assisted Total Coumarins Extraction from Angelica polymorpha Maxim.

关键词:  紫金砂  总香豆素  超声辅助提取  正交试验

Key words:  Angelica polymorpha Maxim.  total coumarins  ultra sonic-assisted extraction  orthogonal test

摘要:

目的研究超声辅助提取紫金砂中总香豆素的最佳提取工艺。方法以总香豆素提取率为指标，考察超声提取温度、提取功率、提取时间、料液比、提取次数对紫金砂总香豆素提取的影响。选用正交试验设计法对紫金砂的超声辅助提取工艺进行优选。结果超声辅助提取紫金砂中总香豆素的最佳工艺为：65 ℃，超声功率90 W，以1∶18的料液比加入丙酮，超声提取50 min，提取4 次。结论通过验证实验, 表明所选的工艺条件可行，在该工艺条件下每克紫金砂平均可提取总香豆素27.4 mg-1。

Abstract:

OBJECTIVE To optimize ultra sonic-assisted extraction process of total coumarins from Angelica polymorpha Maxim.METHODS The extraction rate of total coumarins was used as index and the experimental conditions were investigated, such as solvent, extraction temperature, extration power, extration time, the ratio of solid to liquid and extraction times. The optimal extraction condition was obtained by orthogonal test.RESULTS Optimum technological conditions were determined as follows: 65 ℃, 90 W, 50 min, the ratio of solid to liquid was 1∶18,ultra sonic-assisted extracting for four times.CONCLUSION The process is feasible and useful for extracting total coumarins from Angelica polymorpha Maxim., with average extraction rate of total coumarins as 27.4 mg·g-1.