高效液相色谱柱前衍生化法测定胚宝胶囊中盐酸赖氨酸的含量

Determination of L-lysine Hydrochloride Content in Peibao Capsule by Pre-column Derivation HPLC

关键词:  胚宝胶囊  盐酸赖氨酸  高效液相色谱柱前衍生法

Key words:  peibao capsule  L-lysine hydrochloride  pre-column derivation HPLC

摘要:

目的建立高效液相色谱柱前衍生化法测定胚宝胶囊中盐酸赖氨酸的含量。 方法采用2,4-二硝基氟苯为衍生化试剂进行柱前衍生，色谱柱为Zorbax SB－C18（250 mm×4.6 mm，5 μm），流动相为乙腈-0.04 mol·L-1磷酸二氢钾水溶液（40∶60），检测波长为360 nm，流速为1.0 mL·min-1。结果盐酸赖氨酸在0.12～5.74 μg内线性关系良好，相关系数r＝0.999 9。平均回收率为99.55 %，（RSD=0.48 %，n＝7）。结论该方法简单、快速、重复性好，可作为胚宝胶囊的质量控制方法。

Abstract:

OBJECTIVE To establish a method for the determination of L-lysine hydrochloride content in Peibao capsule by pre-column derivation HPLC.METHODS 2,4-DNFB was used as a derivatization reagent and separation of DNP-amino acid derivatives was performed by HPLC with Zorbax SB－C18 column(250 mm×4.6 mm，5 μm). The mobile phase was consisted of acetonitrile and 0.04 mol·L-1KH2PO4 water solution(40∶60). The flow rate was 1.0 mL·min-1with the wavelength at 360 nm. RESULTS L-lysine hydrochloride showed a good linear relationship at a range of 0.12-5.74 μg,r＝0.999 9. The average recovery was 99.55% with RSD=0.48 %(n＝7).CONCLUSION The method is simple, rapid, accurate and reliable, and it can be applied to control the quality of Peibao capsule.