1,2,3-三磷酸肌醇对四氧嘧啶型糖尿病小鼠降血糖作用研究

Studies on the Hypoglycemic Activity of 1,2,3-inositol Triphosphate in Diabetes Mellitus Mice Induced by Alloxan

关键词:  植酸  1,2,3-三磷酸肌醇  降血糖  四氧嘧啶

Key words:  phytic acid  1,2,3-inositol triphosphate  hypoglycemic activity  alloxan

摘要:

目的研究1,2,3-三磷酸肌醇\[Ins(1,2,3)P3\]对四氧嘧啶所致糖尿病小鼠的降血糖作用。方法用小麦麸皮植酸酶部分水解植酸，水解产物经717型阴离子交换树脂吸附，0.05～0.7 mol·L-1盐酸梯度洗脱分离得到Ins（1,2,3）P3。将四氧嘧啶导致的糖尿病小鼠随机分成5组,每天分别ip生理盐水、10、50、100 mg·kg-1·d-1的Ins（1,2,3）P3钠盐、盐酸苯乙双胍70 mg·kg-1·d-1。连续注射12 d，末次空腹眼眶取血测血糖。结果Ins（1,2,3）P3可降低四氧嘧啶所致糖尿病小鼠的血糖值，在浓度为50 mg·kg-1·d-1与100 mg·kg-1·d-1剂量时，显著降低糖尿病小鼠血糖水平，对正常小鼠血糖无影响。Ins(1,2,3)P3可提高四氧嘧啶型糖尿病小鼠及正常小鼠的胸腺与脾脏指数，抵抗四氧嘧啶引起的小鼠胸腺、胰腺萎缩。结论Ins（1,2,3）P3对糖尿病小鼠具有降血糖作用。

Abstract:

OBJECTIVE To investigate the effects of 1,2,3-inositol triphosphate [Ins(1,2,3)P3] on the blood glucose of Alloxan-diabetic mice. METHODS Phytic acid was partially hydrolyzed by phytase from wheat bran. The partial hydrolyzed solutions were pumped into a column containing 717 strong anion-exchange resin, then delivered a linear gradient of 0.05-0.7 mol·L-1 HCl giving Ins(1,2,3)P3. Alloxan-induced diabetic mice were divided into 5 groups randomly, and intra-peritoneal injected with saline,10， 50, 100 mg·kg-1·d-1 Ins(1,2,3)P3, 70 mg·kg-1·d-1 PHF respectively for 12 days successively. Fasting orbital blood samples were collected for measurement of glucose at the end of the experiment. RESULTS Ins(1,2,3)P3 was found to reduce blood glucose level in alloxan-diabetic mice markedly at doses of 50,100 mg·kg-1·d-1, but no effect on normal mice. Compared with the model control group, Ins(1,2,3)P3 had the ability to raise the thymus and spleen indexes of diabetic mellitus mice and normal mice,inhibiting the atrophy of thymus and pancreas of the diabetic mice induced by alloxan. CONCLUSION Ins(1,2,3)P3 has good hypoglycemic effect on Alloxan-diabetic mice.