HPLC测定复方乙酰水杨酸片中3组分的含量

Determination of Three Components in Compound Aspirin Tablets by HPLC

关键词:  高效液相色谱法  复方乙酰水杨酸片  乙酰水杨酸  咖啡因  非那西丁

Key words:  HPLC  compound aspirin tablets  acetyl salicylic acid  caffeine  phenacetin

摘要:

目的同时测定复方乙酰水杨酸片中乙酰水杨酸、咖啡因、非那西丁的含量。方法采用HPLC，色谱柱：Symmetry C18柱（4.6 mm×150 mm,5 μm），流动相为pH6.5的磷酸二氢钾缓冲液-乙腈（78∶22），流速为1.0 mL·min-1，检测波长为270 nm。结果3组分分别在8.80～70.42 μg· mL-1（r=0.999 9）、1.42～11.34 μg· mL-1(r=0.999 9)、6.12～48.92 μg· mL -1 (r=0.999 9)内成良好线性关系，平均回收率分别为101.2%（RSD=0.7%，n=6）、99.1%（RSD=0.8%，n=6）、100.3%（RSD=0.7%，n=6）。结论本法可不经分离，直接测定复方乙酰水杨酸片中3组分的含量。

Abstract:

OBJECTIVE To determine acetyl salicylic acid,caffeine and phenacetin in compound aspirin tablets. METHODS The chromatographic conditions included Symmetry C18column（4.6 mm×150 mm, 5 μm）.The mobile phase was phosphate salt buffer(pH6.5)-acetonitrile(78∶22).The flow rate was 1.0 mL·min-1.The detection wavelength was 270 nm. RESULTS Acetyl salicylic acid,caffeine and phenacetin had good linear relationship in the range of 8.80-70.42 μg· mL-1（r=0.999 9），1.42-11.34μg· mL-1(r=0.999 9) and 6.12-48.92 μg· mL-1 (r=0.999 9).Their recoveries were 101.2%（RSD=0.7%，n=6），99.1%（RSD=0.8%，n=6） and 100.3%（RSD=0.7%，n=6）,respectively.CONCLUSION This method could be used for the determination of three components in compound aspirin tablets without separation.