离子对HPLC同时测定5种氟喹诺酮类药物

Separation and Determination of Five Fluoroquinolone Antibiotics by Ion Pair-HPLC

关键词:  离子对高效液相色谱法  依诺沙星  环丙沙星  氟罗沙星  诺氟沙星  司帕沙星

Key words:  ion pair-HPLC  enoxacin  ciprofloxacin  fleroxacin  norfloxacin  sparfloxacin

摘要:

目的建立一种离子对高效液相色谱快速分离和测定依诺沙星、环丙沙星、氟罗沙星、诺氟沙星、司帕沙星的方法。方法采用Hypersil ODS2柱（4.6 mm×250 mm，5 μm），流动相为11 mmol·L-1四丁基溴化铵溶液(含0.5%三乙胺)-乙腈（96∶4）用冰醋酸调pH为4.2，流速1.0 mL·min-1，检测波长280 nm。结果在20 min内5种氟喹诺酮类药物（fluoroquinolones，FQS）分离完全，线性范围分别为依诺沙星和诺氟沙星为4～100 μg·mL-1，环丙沙星和氟罗沙星为5～90 μg·mL-1，司帕沙星为8～100 μg·mL-1（r>0.999 4），检测限依诺沙星和诺氟沙星为0.8 μg·mL-1，环丙沙星为1.0 μg·mL-1，氟罗沙星为0.9 μg·mL-1，司帕沙星为1.2 μg·mL-1。结论本方法简便快速、准确、重复性好。

Abstract:

OBJECTIVE To establish an ion pair-HPLC with fast separation and determination of enoxacin,ciprofloxacin,fleroxacin,norfloxacin and sparfloxacin. METHODS Hypersil ODS2collumn (4.6 mm×150 mm，5 μm ) was used with 11 mmol·L-1 tetrabutylammonium bromide solution (0.5%triethylamine)-acetonitrile (96∶4) pH4.2 used by acetic acid as the mobile phase with a flow rate of 1.0 mL·min-1 and UV wavelength was set at 280 nm. RESULTS Five fluoroquinolones have been separated completely in 20 minutes.The linear range of enoxacin and norfloxacin was 4-100 g·mL-1, for ciprofloxacin and fleroxacin it was 5-90 g·mL-1，for sparfloxacin it was 8-100 μg·mL-1(r> 0.999 4).The detection limit of enoxacin and norfloxacin was 0.8 μg·mL-1,for ciprofloxacin it was 1.0 μg·mL-1, for fleroxacin it was 0.9 μg·mL-1，for sparfloxacin it was 1.2 μg·mL-1.CONCLUSION The method is simple, rapid, accurate and reproducible.