槲皮苷防治溃疡性结肠炎的药效学研究

Protective Effect of Quercitrin Against Ulcerative Colitis in Rat

关键词:  槲皮苷  溃疡性结肠炎  抗炎  镇痛  止泻

Key words:  Quercitrin  ulcerative colitis  anti-inflammatory  analgesic  anti-diarrhea

摘要:

目的观察槲皮苷防治溃疡性结肠炎及抗炎、镇痛、止泻等药效。 方法以醋酸灌肠复制溃疡性结肠炎模型，大鼠从造模前2 d至造模后6 d ig给予槲皮苷（50,200 mg·kg-1·d-1），取结肠粘膜进行组织病理学检查以及丙二醛（MDA）、髓过氧化物酶（MPO）测定。小鼠ig给予槲皮苷（75,300 mg·kg-1·d-1）4 d后测定腹腔毛细血管通透性、醋酸ip引起扭体反应以及番泻叶煎剂ig引起腹泻潜伏期及次数变化。 结果槲皮苷减轻溃疡性结肠炎大鼠结肠病变程度，降低结肠粘膜组织MDA及MPO水平（P<0.05）；抑制醋酸引起的小鼠腹腔毛细血管通透性增加，减少扭体次数（P<0.05）；对腹泻潜伏期及腹泻次数无显著影响（P>0.05）。 结论槲皮苷对大鼠溃疡性结肠炎具有防治作用，并有抗炎、镇痛作用。

Abstract:

OBJECTIVE To investigate the protective effect of Quercitrin against ulcerative colitis in rats and anti-inflammatory, analgesic and anti-diarrhea effects of Quercitrin in mice. METHODS Rats treated with oral Quercitrin （50,200 mg·kg-1·d-1） for 2 days before and 6 days after ulcerative colitis induced by acetic acid. Colonic pathological changes, tissue malondialdehyde (MDA) and myeloperoxidase (MPO) levels were determined. Anti-inflammatory, analgesic and anti-diarrhea effects of Quercitrin （75,300 mg·kg-1·d-1） treated with oral for 4 days in mice were determined by penetrability of abdominal capillary vessel assay, acetic acid-induced writhing assay and senna-induced diarrhea assay respectively. RESULTS Quercitrin improved colonic pathological changes and reduced MDA and MPO levels in ulcerative colitis rats (P<0.05). Quercitrin suppressed the increased penetrability of abdominal capillary vessel induced by acetic acid (P<0.05); inhibited the writhing response induced by acetic acid (P<0.05); but had no significant effect on diarrhea induced by senna in mice (P>0.05). CONCLUSION Quercitrin has the protective effect against ulcerative colitis in rats and has anti-inflammatory and analgesic effects in mice.