HPLC测定红药片中三七皂苷R1和人参皂苷Rg1的含量

Determination of Notoginsenoside R1 and Ginsenoside Rg1 in Hongyao Tablet by HPLC

关键词:  红药片  高效液相色谱法

Key words:  Hongyao tablets  HPLC

摘要:

目的建立红药片中三七皂苷R1和人参皂苷Rg1的HPLC测定方法。方法采用Agilent1100 DAD高效液相色谱仪,Discovery C18色谱柱(25cm×4.6mm,5μm),乙腈-0.1%磷酸(20∶80)为流动相,检测波长为203nm,流速1mL·min-1。结果三七皂苷R1在1.216～9.728μg(r=0.9995),人参皂苷Rg1在3.036～15.180μg(r=0.9991)内线性关系良好。三七皂苷R1平均回收率为98.6%,RSD=1.7%;人参皂苷Rg1平均回收率为101.0%,RSD=1.7%。结论该法简便、快速、准确,适用于红药片中三七皂苷R1和人参皂苷Rg1的含量定量测定,有利于控制红药片质量。

Abstract:

OBJECTIVE To establish a HPLC method for simulataneous determination of Notoginsenoside R1 and Ginsenoside Rg1 in Hongyao Tablets. METHODSThe HPLC was conducted on Discovery C18 column(25 cm×4.6 mm,5 μm)with acetonitrile-0.1% phosphoric acid solution (20∶80) as the mobile phase.The detection wavelength was 203 nm,and flow rate was 1 mL·min-1. RESULTSThere were good linear relations of Notoginsenoside R1 within 1.216-9.728 μg·mL-1 (r=0.999 5) and Ginsenoside Rg1 within 3.036-15.180 μg·mL-1 (r=0.999 1).The average recovery rate of Notoginsenoside R1 was 98.6%,RSD=1.7%; and Ginsenoside Rg1 was 101.0%,RSD=1.7%. CONCLUSIONThe method is simple, rapid and accurate for the determination of Notoginsenoside R1 and Ginsenoside Rg1 in Hongyao Tablets,and it is expected for the quality control of Hongyao tablets.