河南不同产地夏枯草的质量对比分析

The Quality Analysis of Defferent Areas of Henan′s Spica Prunellae

关键词:  夏枯草  熊果酸  齐墩果酸  质量分析

Key words:  Spica Prunellae  ursolic acid  oleanolic acid  quality analysis

摘要:

目的通过采用高效液相法测定河南不同产地夏枯草中熊果酸、齐墩果酸的含量来比较夏枯草质量的优劣，为夏枯草的质量控制方法提供一定的依据。方法测定熊果酸、齐墩果酸色谱条件为Betasil C18(250 mm×4.6 mm,5 μm)；流动相：磷酸二氢钠缓冲液[取磷酸二氢钠7.8 g加水至1 000 mL]-甲醇（14∶86）；流速：0.8 mL·min-1；柱温：25 ℃；检测波长：210 nm；结果熊果酸在0.554～5.34 μg内线性关系良好，r=0.999 7，平均回收率为97.45%，RSD为0.92%。齐墩果酸在0.28～2.24 μg内线性关系良好，r=0.999 4，平均回收率为97.61%，RSD为0.87%。结论该方法简单、准确并且专属性强。

Abstract:

OBJECTIVE To establish an HPLC method for the determination of ursolic acid and oleanolic acid in different area of Henan′s Spica Prunellae. METHODS The ursolic acid and oleanolic acid separation was performed on a column: Betasil C18(250 mm×4.6 mm,5 μm) column, mobile phase was natrium dihydrogen phosphate solution (7.8 g→1000 mL)-methanol (14∶86), the flow rate was 0.8 mL·min-1, the column temperature was 25 ℃, the detective wavelength was 210 nm. RESULTS The ursolic acid calibration curve showed good linearity in the range of 0.554-5.34 μg(r=0.999 7), the average recovery was 97.45%(RSD=0.92%). The oleanolic acid calibration curve showed good linearity in the range of 0.28-2.24 μg (r=0.999 4), the average recovery was 97.61%(RSD=0.87%). CONCLUSION The method is simple,accurate and sensitive.