小连翘内生放线菌YBL-02菌株的初步鉴定和发酵产物分离

Taxonomy, Fermentation and Products Characterization of Actinomycete Strain YBL-02 from Hyperioums Sampsonii Hance

关键词:  内生菌  小连翘  放线菌  发酵

Key words:  endophytic fungi  hyperioums sampsonii hance  actinomyces  fermentation

摘要:

目的 对小连翘(Hyperioums Sampsonii Hance.) 内生放线菌YBL-02菌株的进行分类、发酵及产物的提取和初步鉴定。方法 根据形态学，培养特征等初步鉴定YBL-02菌株，并采用薄层色谱和红外光谱等对YBL-02发酵产物进行分离和初步鉴定。结果 根据菌株的形态学，培养特征等初步将YBL-02菌株归属为链霉菌属。对YBL-02菌株发酵产物进行分离纯化，最后得到具有活性的片状白色结晶体，测其熔点为117～119 ℃。对该物质进行红外光谱分析后可得出为一种酰胺类化合物。结论 YBL-02菌株是一种链霉菌，其发酵产物中活性成分为一种酰胺类化合物。

Abstract:

OBJECTIVE Taxonomy of strain YBL-02 isolated from the roots of Hyperioums Sampsonii Hance was studied. And the fermentative products of strain YBL-02 were collected and identified primarily.METHODS The characteristics of substrate mycelium , aerial mycelium and growth on the different culture media were used to identify the strain YBL-02. Some techniques, such as thin-layer chromatography, infra-red spectrum, were used to collected and identified the products of fermentation of strain YBL-02.RESULTS Strain YBL-02 was an actinomycete by the primary taxonomic study. The fermentative products of strain YBL-02 were a kind of lamellar white crystalline form. The melting point of the crystalline form was 117-119℃.It was identified as a kind of chemical compound containing amide groups. CONCLUSION Strain YBL-02 is an actinomycete，and its fermentative products were a kind of chemical compounds containing amide groups.