HPLC测定多柔比星壳寡糖硬脂酸胶团中药物的含量

HPLC Determination of Doxorubicin Concentration in Chitosan Oligosaccharide-stearic Acid Micelles

关键词:  多柔比星  高效液相色谱法  壳寡糖硬脂酸嫁接物胶团

Key words:  doxorubicin  HPLC  chitosan oligosaccharide-stearic acid micelles

摘要:

目的 采用高效液相色谱法测定壳寡糖硬脂酸嫁接物胶团（CSO-SA）中的多柔比星（DOX）的含量。方法 采用Shim-pack VP-ODS色谱柱（150 mm ×4.6 mm，5 μm），以0.01 mol·L-1的十二烷基硫酸钠溶液-乙腈-甲醇（50∶50∶6）为流动相，内含0.6‰（v/v）的磷酸，流速1.0 mL·min-1，检测波长254 nm，柱温25 ℃。结果 多柔比星在0.4～8 μg·mL-1时，浓度与峰面积呈良好的线性关系，Y＝52.926X-3.761（r＝0.999 9，n=3），定量限为（0.05±0.014）μg·mL-1（S/N>10，n=3）。高、中、低三个浓度的日内RSD为1.82%、0.86%、0.65%，日间RSD为1.73%、0.88%、0.75%，平均加样回收率为(99.38±1.22)%。结论 本测定方法操作简便，结果准确。

Abstract:

OBJECTIVE An HPLC analysis method was established for the determination of doxorubicin (DOX) concentration in chitosan oligosaccharide-stearic acid (CSO-SA) micelles．METHODS The segregation and determination of samples were performed on a Shim pack VP-ODS (150 mm ×4.6 mm，5 μm) column with a mobile phase consisting of 0.01 mol·L-1 sodium dodecyl sulfate-methanol-acetonitrile (50∶50∶6) containing 0.6‰ phosphoric acid at a flow-rate of 1.0 mL·min-1，UV detector was set at 254 nm and column temperature was 25 ℃.RESULTS There was good linear relationship within the range of 0.4～8 μg·mL-1 of DOX，Y＝52.926X-3.761（r＝0.999 9，n=3）．The quantification limit was (0.05±0.014) μg·mL-1(S/N>10，n=3). The RSDs of within-day were 1.82%,0.86%,0.65%, and the RSDs of between-day were 1.73%,0.88%,0.75%，respectively.The average recovery was(99.38 ±1.22)%.CONCLUSION The HPLC methods are simple and accurate for the doxorubicin concentration in micellar delivery system．