胶束电动毛细管色谱法测定头孢克肟的含量

Determination of Cefixime by Micellar Electrokinetic Capillary Chromatography

关键词:  胶束电动毛细管色谱  头孢克肟  乙酰苯胺  含量测定

Key words:  micellar electrokinetic capillary chromatography  cefixime  acetanilide  content determination

摘要:

目的建立测定头孢克肟含量的胶束电动毛细管色谱方法。方法采用非涂渍石英毛细管51 cm×75 μm；运行缓冲液为含100 mmol·L-1十二烷基硫酸钠的磷酸盐缓冲液（70 mmol·L-1，pH 7.0）;压力进样5 s,分离电压15 kV,柱温25 ℃，检测波长254 nm,选择乙酰苯胺为内标。结果头孢克肟在6.08～777.98 μg·mL-1内，线性关系良好（r=0.999 3）。头孢克肟的最低检测限为1.03 μg·mL-1，最低定量限为3.43 μg·mL-1；含量测定结果与HPLC所得结果没有显著性差异。结论胶束电动毛细管色谱法分析头孢克肟的含量，方法准确、简便、灵敏，与HPLC具有互补性。

Abstract:

OBJECTIVE To develop a method of micellar electrokinetic capillary chromatography for determination of cefixime. METHODS The uncoated fused-silica capillary (51 cm×75 μm) was used.The running buffer composed of 70 mmol·L-1 phosphate buffer containing 100 mmol·L-1 sodium dodecylsulphate(pH 7.0).The sample was injected to the column by pressure (50 mbar) for 5 s.The separation voltage was 15 kV and the capillary temperature was 25℃.The detection wavelength was set at 254 nm with acetanilide as internal standard. RESULTS The calibration curve was linear within the range of 6.08-777.98 μg·mL-1 for cefixime (r=0.999 3).The limit of detection and limit of quantitation were 1.03 μg·mL-1 and 3.43 μg·mL-1 respectively.Statistical analysis by t-test showed no significant differences between the results obtained by micellar electrokinetic capillary chromatography (MECC) and by high performance liquid chromatography (HPLC). CONCLUSION The MECC method developed was accurate ,simple and sensitive,and could be complement to HPLC.