HPLC测定两面针药材中氯化两面针碱的含量

Determination of Nitidine Chloride in Zanthoxylum nitidum (Roxb．) DC．by HPLC

关键词:  两面针  氯化两面针碱  高效液相色谱法

Key words:  Zanthoxylum nitidum (Roxb.) DC  nitidine chloride  HPLC

摘要:

目的建立高效液相色谱法测定药材中氯化两面针碱含量的方法。方法采用色谱柱为SymmetryshielyTM RP18 柱（4.6 mm×250 mm, 5 μm），流动相为乙腈-0.04 mol·L-1醋酸钠溶液（用醋酸调节pH为6.5），检测波长为270 nm。结果　氯化两面针在0.25～5.0 μg内线性关系良好。平均回收率为99.6%，RSD为1.49% 。结论该方法简单准确，重复性好，可用于两面针药材的质量控制。

Abstract:

OBJECTIVE To establish an HPLC method for the determination of nitidine chloride in Zanthoxylum nitidum (Roxb.) DC. METHODS The method contained a SymmetryshielyTM RP18 (4.6 mm×250 mm, 5 μm) as column, with acetonitrile-0.04 mol·L-1 NaAc（adjust pH to 6.5 with Hac）as mobile phase, UV detection wavelength at 270nm. RESULTS The calibration curve of nitidine chloride was linear between 0.25-5.0 μg.The average recovery was 99.6%, RSD=1.49%. CONCLUTION This method was proved to be simple, rapid, accurate and can be used for the quality control of Zanthoxylum nitidum（Roxb.）DC.