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引用本文:陈晓红.HPLC-FLD同时测定全血中加替沙星和莫西沙星含量[J].中国现代应用药学,2009,(5):412-414.
CHEN Xiaohong.Determination of Gatifloxacin and Moxifloxacin in Whole Blood by HPLC Coupled with Fluorescence Detection[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(5):412-414.
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HPLC-FLD同时测定全血中加替沙星和莫西沙星含量
陈晓红
作者单位
陈晓红  
摘要:
目的建立一种灵敏、可靠的全血中加替沙星和莫西沙星的高效液相色谱荧光检测方法。方法样品经磷酸盐缓冲液提取后,用Waters Oasis MAX 小柱进行净化,甲醇-乙腈-0.2%甲酸(15∶15∶70)为流动相,采用Cloversil - C18柱(3.0 mm ×150 mm, 5 μm)分离,荧光检测波长:λex 288 nm和λem 493 nm,内标法定量。结果加替沙星和莫西沙星在15.0~240.0 μg·L-1内呈良好线性,方法回收率在97.7%~101.3% 之间,日内RSD<6.0%,其定量限为15.0 μg·L-1。结论本方法简便、灵敏、干扰少,特异性好,能满足血液中加替沙星和莫西沙星的检测要求。
关键词:  高效液相色谱-荧光法  全血  加替沙星  莫西沙星
DOI:
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基金项目:
Determination of Gatifloxacin and Moxifloxacin in Whole Blood by HPLC Coupled with Fluorescence Detection
CHEN Xiaohong
Abstract:
OBJECTIVE To develop a sensitive and accurate method for the determination of gatifloxacin and moxifloxacin in whole blood by high-performance liquid chromatography coupled with fluorescence detection(HPLC-FLD). METHODS After gatifloxacin and moxifloxacin in whole blood were extracted by phosphate buffer, the sample was cleaned with solid-phase extraction (SPE) using Oasis MAX cartridges.The separation was performed on Cloversil - C18 column(3.0 mm ×150 mm, 5 μm)using the mobile phase consisting of methanol-acetonitrile-0.2% formic acid(15∶15∶70).Detection was carried out on a fluorescence detection at λex 288 nm and λem 493 nm. RESULTS Calibration curves were linear within the range of 15.0-240.0 μg·L-1, and the extraction recoveries were from 97.7% to 101.3%, the RSDs were less than 6.0%.The limits of quantification were found to be 15.0 μg·L-1.CONCLUSION This method is found to be simple,sensitive,little interferential and good specificity for the determination of gatifloxacin and moxifloxacin in whole blood.
Key words:  HPLC-FLD  whole blood  gatifloxacin  moxifloxacin
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