五味子提取物定性鉴别和含量测定研究

Identification and Determination of Qualitative Research for Extraction of Schisandra Chinensis(Turcz.) Baill.

关键词:  五味子  紫外可见分光光度法  高效液相色谱法

Key words:  Schisandra Chinensis (Turcz.) Baill.  UV-visible spectrophotometry  HPLC

摘要:

目的建立五味子提取物的定性鉴别和含量测定标准。方法采用TLC鉴别五味子；采用紫外可见分光光度法，570 nm波长，测定五味子木脂素含量；采用RP-HPLC，以甲醇-乙腈-水(1∶1∶1)为流动相，250 nm为检测波长，测定五味子醇甲、五味子甲素、五味子乙素含量。结果供试品色谱中，在与对照品和对照药材色谱相应的位置上，显示相同颜色的荧光斑点；五味子木脂素以乙素计，在0.005 5～0.026 7 mg·mL-1内，线性关系良好，平均回收率101.4%(RSD= 2.5%)；五味子醇甲在0.275～1.375 μg (r=0.999 7)内、五味子甲素在0.125～0.625 μg (r=0.999 7)内、五味子乙素在0.160～0.800 μg (r=0.999 7)内，线性关系良好，平均回收率分别为97.9%(RSD=1.4%)、99.0%(RSD=1.9%)、98.2%(RSD=1.9%)。结论采用方法简便、准确、快速，可对五味子提取物进行有效的质量控制。

Abstract:

OBJECTIVE To establish the identification and determination of qualitative standard for extraction of Schisandra Chinensis(Turcz.) Baill. METHODS Fructus Schisandra Chinensis was identified by TLC. The determination of lignan was performed by UV-visible spectrophotometry. The determination of effective eomponents were performed by HPLC with the mobile phase consisted of methanol-acetonitrile-water(1∶1∶1)，and the wavelength was set at 250 nm. RESULTS The tested products in chromatography，in contrast with the reference substance and medicine by the corresponding position，showed the same color of fluorescent spots. Schisandra lignans of the γ-Schisandrin，a good linearity of γ-Schisandrin was obtained ranged from 0.005 5-0.026 7 mg·mL-1，and the average recovery was 101.4%(RSD=2.5%). Three good linearities of Schisandrin，Deoxyschizandrin and γ-Schisandrin were obtained ranged from 0.275-1.375 μg (r=0.999 7)，0.125-0.625 μg(r=0.999 7) and 0.160-0.800 μg(r=0.999 7)，and the average recoveries were 97.9%(RSD=1.4%)，99.0%(RSD=1.9%) and 98.2%(RSD=1.9%). CONCLUSION The methods are simple，accurate，and specific，and can be used for the quality control of extraction of Schisandra Chinensis (Turcz.) Baill.