白蚁菌圃抗炎机制的研究

Study of Anti-inflammatory Mechanism About Fungus Garden of Odontotermes Formosanus

关键词:  白蚁菌圃  抗炎机制

Key words:  fungus garden of Odontotermes formosanus  anti-inflammation mechanism

摘要:

目的 探讨白蚁菌圃抗炎的作用机制。方法 采用角叉菜胶致正常及切除双侧肾上腺小鼠足肿胀;角叉菜胶致大鼠胸膜炎；蛋清致小鼠足肿胀的炎症模型。分别用酶联免疫吸附法、紫外分光光度法和荧光分光光度法测炎性渗出液中白三烯(LTB4)、前列腺(PGE2)、5-羟色胺(5-HT)及组胺的水平。结果 角叉菜胶致正常组及去肾上腺组小鼠足肿胀炎症组织及血清中PGE2含量与阴性对照组比有显著性差异；在蛋清致足肿胀中的组胺和5-羟色胺的含量与阴性对照组比有显著性差异；在角叉菜胶致胸膜炎大鼠胸腔渗出液中白三烯含量与阴性对照组比有显著性差异。结论 白蚁菌圃的抗炎作用可能与其抑制炎症部位PGE2、LTB4、5-HT及组胺的水平有关，其抗炎作用不依赖下丘脑-垂体-肾上腺皮质系统的调节。

Abstract:

OBJECTIVE In order to study anti-inflammation mechanism of fungus garden of Odontotermes formosa-nus. METHODS Four types of models of the norml mouse and the excision two-sides adrenal gland mouse paw swollen induced by carrageenan, rat pleurisy induced by carrageenom,mouse paw swollen induced by allrumin were set up. The enzyme linked immunosorbent assay, the spectrophotometric and fluorometric method were used separately to measure the leukotriene (LTB4), the prostate gland (PGE2), hydroxy tryptamine (5-HT) and histamine level in the inflammatory percolate. RESULTS There was significant difference of PGE2, 5-HT, LTB4 or histamine between four types of models and the negative control group. CONCLUSION The anti-inflammation function of fungus garden of Odontotermes formosanus may be related to its suppression of PGB2, LTB4, 5-HT and histamine, and does not rely on hypothalamic-pituitary-adrenocortical system.