生长时期施肥种类对柴胡HPLC指纹图谱影响分析

Effect of HPLC Fingerprint of Radix Bupleuri with Different Fertilizers at Different Growth Stages

关键词:  柴胡  高效液相色谱法  指纹图谱

Key words:  Radix Bupleuri.  HPLC  fingerprint

摘要:

目的 建立柴胡HPLC-PDA指纹图谱分析方法。方法 采用高效液相色谱法，色谱柱为SunFireTMC18柱(250 mm×4.6 mm，5 μm)，以乙腈-0.1%磷酸梯度洗脱，检测波长为210 nm，柱温30 ℃。结果 建立了柴胡的HPLC-PDA指纹图谱，确定了15个共有峰，不同施肥柴胡样品指纹图谱与对照指纹图谱相似度均在0.90以上，可以用于柴胡药材的定性鉴别。结论 此方法简单、准确、重复性好，为柴胡的质量控制提供了有效的方法。

Abstract:

OBJECTIVE To establish a method of fingerprint analysis on Radix Bupleuri by HPLC-PDA. METHODS The HPLC method was used with a SunFireTM C18 column (250 m×4.6 mm,5 μm).The mobile phase was acetonitrile-0.1% phosphricacid and the detective wavelength was set at 210 nm with the column temperature of 30℃. RESULTS Establishing the fingerprint of Radix Bupleuri,15 common peaks were found in the HPLC fingerprint of Radix Bupleuri and good similarities with correlation coefficients higher than 0.90 were found in fingerprints between the herbs and standard fin/gerprint, which can be utilized for the identification of Radix Bupleuri. CONCLUSION The method is accuate, simple, and could be used for quality control of Radix Bupleuri.