我院常用抗癫痫药物人血药浓度的测定

Blood Concentration Determination of Anti-epileptic Drugs in Our Hospital

关键词:  高效液相色谱法  苯巴比妥  苯妥英钠  卡马西平  血药浓度监测

Key words:  HPLC  phenobarbital  phenytoin-sodium  carbamazepine  plasma concentration detection

摘要:

目的 探讨建立苯巴比妥、苯妥英钠、卡马西平人血清浓度的测定方法，并应用该方法测定我院患者样本。方法 采用高效液相色谱法(HPLC)，流动相：乙晴-缓冲盐(30∶70)；柱温：37 ℃；检测波长：220 nm；流速：0.7 mL·min-1。含量测定分析采用内标对比法，杂质检查分析采用归一化法。结果 该方法测定苯巴比妥、苯妥英钠、卡马西平的线性范围分别为4.58～75.88 mg·L-1，2.25～35.95 mg·L-1，1.26～20.08 mg·L-1，日内和日间RSD均小于10%；回收率可控制在85%～115%之间。结论 该方法具有良好的准确性、精密性和较高的灵敏度，且简便、快速、稳定。

Abstract:

OBJECTIVE To establish a method to determine the concentration of Phenobarbital(PB), phenytoin-sodium(PT) and carbamazepine(CBZ) in the human plasma，and determine the samples in our hospital with this method. METHODS The high performance liquid chromatography (HPLC) was used. The analysis conditions were as follows: acetonitrile-buffer salt solution (30∶70) as mobile phase, column oven temperature 37℃, wavelength 220 nm and flow rate 0.7 mL·min-1. Internal standard method was developed to analyze the content of drugs, and normalization method was developed to analyze the content of impurity. RESULTS The linear ranges were 4.58-75.88 mg·L-1, 2.25-35.95 mg·L-1 and 1.26-20.08 mg·L-1 for PB , PT and CBZ, respectively. The RSD within-day and between-days were both less than 10.0%. The average rate of recovery can be controlled in 85%-115%. CONCLUSION This method is simple, rapid, stable, with good accuracy, precision and high sensitivity.