HPLC测定灯盏花素缓释胶囊含量

Determination of Scutellarin Brevisapin Sustained-Release Capsules by HPLC

关键词:  灯盏花素缓释胶囊  高效液相色谱法  野黄芩苷  含量测定

Key words:  brevisapin sustained-release capsules  HPLC  scutellarin  content determination

摘要:

目的建立灯盏花素缓释胶囊中野黄芩苷的高效液相色谱法(HPLC)含量测定方法。方法采用高效液相色谱法，C18柱色谱柱(4.6 mm ×150 mm，5 μm)，柱温30 ℃；进样量10 μL，流动相：甲醇-四氢呋喃-0.1%磷酸溶液(14∶14∶72)，流速1.0 mL·min-1；检测波长335 nm。结果野黄芩苷在24.45～293.46 μg·mL-1范围内，峰面积与浓度的线性关系良好(r=0.999 9)，野黄芩苷平均回收率为101.5%，RSD为3.616%。结论该方法简便准确，重现性好，可用于灯盏花素缓释胶囊的质量控制。

Abstract:

OBJECTIVE To develop an HPLC method of scutellarin in brevisapin sustained-release capsules. METHODS C18(4.6 mm ×150 mm, 5 μm) column with temperature of 30 ℃ was used. The mobile phase was the mixed solution of methanol- tetrahydrofuran-0.1% phosphoric acid (14∶14∶72) with the flow rate of 1.0 mL·min-1. Determination wavelength was 335 nm and the sample injected volume was 10 μL. RESULTS A good linearity of scutellarin was in the range of 24.45-293.46 μg·mL-1. The average recovery of scutellarin was 101.5% with RSD=3.616%. CONCLUSION The method is simple, accurate, and can be used for the quality control of brevisapin sustained-release capsules.