| 引用本文: | 石钺，杨帅，李开通.HPLC测定骨骼风痛胶囊中薯蓣皂苷元的含量[J].中国现代应用药学,2009,(8):657-659. |
| SHI Yue, YANG Shuai, LI Kaitong.Determination of Diosgenin in Gugefengtong Capsule by RP-HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(8):657-659. |
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| 摘要: |
| 目的 建立骨骼风痛胶囊中薯蓣皂苷元的含量测定方法。方法 采用HPLC，色谱柱为Hypersil ODS2(4.6 mm×250 mm，5 μm)；流动相为甲醇-水(93∶7)；检测波长203 nm。结果 薯蓣皂苷元在0.772 8～3.864 0 μg内与峰面积呈良好的线性关系，回归方程为Y=443 254X＋18 856，r=0.999 1；平均回收率为97.87%，RSD为1.71%。结论 本法可用于骨骼风痛胶囊的质量控制。 |
| 关键词: 骨骼风痛胶囊 薯蓣皂苷元 高效液相色谱法 |
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| Determination of Diosgenin in Gugefengtong Capsule by RP-HPLC |
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SHI Yue, YANG Shuai, LI Kaitong
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| Abstract: |
| OBJECTIVE To establish an HPLC method for the determination of diosgenin in Gugefengtong capsule. METHODS diosgenin was separated on Hypersil ODS2 (4.6 mm 250 mm, 5 μm) column and detected at 203 nm, with methanol-water (93∶7) as mobile phase. RESULTS There was good linearity from 0.772 8 to 3.864 0 μg (Y= 443 254X＋18 856, r=0.999 1) in separation of diosgenin. The average recovery was 97.87% with RSD of 1.71%. CONCLUSION This method provides the reference to establish quality standard for Gugefengtong capsule. |
| Key words: gugefengtong capsule diosgenin RP-HPLC |
