HPCE同时测定黄强软膏中盐酸小檗碱和泼尼松的含量

Simultaneous Determination of Berberine and Prednisone in Huangqiang Ointment by HPCE

关键词:  高效毛细管电泳  黄强软膏  盐酸小檗碱  泼尼松

Key words:  HPCE  huangqiang ointment  berberine  prednisone

摘要:

目的建立简便、快速的同时测定黄强软膏中盐酸小檗碱和泼尼松含量的高效毛细管电泳。方法以未涂层弹性石英毛细管(41 cm×50 μm，有效分离长度33 cm)为分离通道，30 mmol·L-1四硼酸钠(pH 11. 0)-甲醇(7∶3)为运行缓冲溶液，进样时间10 s，运行电压15 kV。紫外检测波长为238 nm。结果盐酸小檗碱在32.0～512 μg·mL-1内线性关系良好(r=0.999 9)，平均回收率为99. 4%，RSD为1. 2%；泼尼松在16.0～256 μg·mL-1内线性关系良好(r=0.999 0)，平均回收率为99. 8%，RSD为1. 4%。结论本法简便、快速，准确，为黄强软膏的质量控制提供了新的检测手段。

Abstract:

OBJECTIVE To establish a simple and fast method for the determination of berberine and prednisone simultaneously in Huangqiang ointment by HPCE. METHODS An uncoated fused-silica capillary column (41 cm 50 μm, effective length: 33 cm) was used, with a buffer solution of 30 mmol·L-1 sodium borate (pH 11. 0)-methanol (7∶3), inject time of 10 s and separation voltage of 15 kV. The detection wavelength was 238 nm. RESULTS The standard curve of berberine was linear over the range of 32.0-512 μg·mL-1 (r=0. 999 9), and the average recovery was 99. 4% with RSD= 1.2%. The standard curve of prednisone was linear over the range of 16.0-256 μg·mL-1 (r=0.999 0), and the average recovery was 99. 8% with RSD=1. 4%. CONCLUSIONS The HPCE method is simple, rapid and accurate. It provides a new detecting method for the quality control of Huangqiang ointment.