齐多夫定肉豆蔻酯脂质体与齐多夫定对实验动物毒性作用比较研究

Comparative Study of Toxicity of Azidothymidine Myristate Liposome and Azidothymidine for Kunming Mice and Beagle Dogs

关键词:  齐多夫定  脂质体  毒性

Key words:  Azidothymidine  liposome  toxicity

摘要:

目的研究齐多夫定肉豆蔻酯脂质体与齐多夫定对实验动物的毒性作用。方法采用昆明种小鼠，观察两种剂型药物的急性LD50。将昆明种小鼠分成3组，观察两种剂型药物对小鼠的行为活动、体重以及脏器系数的影响。采用Bagle犬分成3组，观察两种剂型药物对犬的行为活动、体重、脏器系数、血液学指标、血清生化值、脏器病理的影响。结果AZT组的LD50为12.50 mg·kg-1，AZT-ML组的LD50为20.30 mg·kg-1。AZT-ML组小鼠的行为活动基本正常，其体重、脏器系数的改变较AZT组明显减轻。AZT-ML组犬的行为活动基本正常、其体重、脏器系数、血液学指标、血清生化值、脏器病理的改变较AZT组明显减轻。结论与AZT相比， AZT-ML对实验动物机体的损害及毒性作用显著降低。

Abstract:

OBJECTIVE To detect and compare the toxicity action of Azidothymidine myristate liposome (AZT-ML) and Azidothymidine(AZT) in Kunming mice and Beagle dogs. METHODS Detecting acute LD50 of two drugs by tail interavenous injection in mice. The mice were divided into 3 groups to observe the effect of action, body weight and organ coefficient. Another, the dogs were divided into 3 groups to observe the effect of action, body weight, organ coefficient, blood physiological index, serum biochemical parameters and organ pathology. RESULTS LD50 of AZT was 12.50 mg·kg-1, LD50 of AZT-ML was 20.30 mg·kg-1. Action of mice was normal, the change of body weight, organ coefficient of mice, AZT-ML group had significant decreases by comparing with AZT group. Action of dogs was normal, the change of action, body weight, organ coefficient, blood physiological index, serum biochemical parameters and organ pathology of dogs, AZT-ML group had significant decreases by comparing with AZT group. CONCLUSION To compare with AZT, AZT-ML can significantly reduce impairment of organism and toxicity of laboratorial animal.