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| 摘要: |
| 目的对白首乌C21甾苷元告达庭甾苷元和开德甾苷元(Ⅱ) 实现C11α-羟基化，要求副产物少，收率高。方法采用黑根霉(Rhizopus nigricans) 和犁头霉(Absidia sp.)两种微生物在水－正丁醇双相体系中同步转化的方法，羟化反应中控制值pH在5.0～6.0，于30 ℃温度下振荡培养70 h。结果产物经IR、MS、NMR分析确认是C11α-羟基化了的告达庭甾苷元(Ⅲ)和开德甾苷元(Ⅲ) ，副产物少，收率达70.1%。结论用黑根霉(Rhizopusnigricans) 和犁头霉(Absidia sp.)两种微生物在水－正丁醇双相体系中同步转化的方法是实现C11α-羟基化的有效方法。 |
| 关键词: 黑根霉 犁头霉 C11α-羟基化 同步转化 |
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| C11 α-hydroxylation of C21 Steroidal Aglycones in Cynanchum Auriculatum by Microbial Simultaneously Transformation |
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LI Yushan, HE Yan
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| Abstract: |
| OBJECTIVE Two species of C21 steroidal aglycones of Caudatin steroidal aglycones and Kaider steroidal aglycones, which were extracted from Cynanchum auriculatum, could be C11 α-hydroxylated with low by-product content and high-yield objectives. METHODS Rhizopus nigricans and Absidia sp. Were used to synchronized transformation and by shaking cultivation for 70 hours at 30 ℃ in water/butanol two-phase system with pH=5.0-6.0. RESULTS The products were confirmed to be C11 α-hydroxylated Caudatin steroidal aglycones and Kaider steroidal aglycones by IR, MS, and NMR. The results also indicated that by-product content was low and the yield of objectives was up to 70.1%. CONCLUSIONS The method was high effective for C11 α-hydroxylation of C21 steroidal aglycones in water/butanol two-phase system by Rhizopus nigricans and Absidia sp |
| Key words: Rhizopus nigricans Absidia sp C11 α-hydroxylation synchronous |
